Generation of genome-edited chicken and duck lines by adenovirus-mediated in vivo genome editing

Joonbum Lee, Dong Hwan Kim, Madeline C. Karolak, Sangsu Shin, Kichoon Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Conventional avian genome editing is mediated by isolation, culture, and genome editing of primordial germ cells (PGCs); screening and propagating the genome-edited PGCs; and transplantation of the PGCs into recipient embryos. The PGC-mediated procedures, however, are technically difficult, and therefore, the conventional method has previously been utilized only in chickens. Here, we generated germline mosaic founder chicken and duck lines without the PGC-mediated procedures by injecting an adenovirus containing the CRISPR-Cas9 system into avian blastoderms. Genome-edited chicken and duck offspring produced from the founders carried different insertion or deletion mutations without mutations in the potential off-target sites. Our data demonstrate successful applications of the adenovirus-mediated method for production of genome-edited chicken and duck lines.

Original languageEnglish
Article numbere2214344119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number45
DOIs
StatePublished - 8 Nov 2022

Keywords

  • adenovirus
  • chicken
  • CRISPR-Cas9
  • duck
  • genome editing

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