Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor

Sun Kwon Mo; Chul Koo Bon; Ryul Choi Bok; Yoon Yub Park; Man Lee Young; Suk Suh Hun; Sik Park Young; Taek Lee Hoon; Jin Hoi Kim; Yeol Roh Ji; Nam Hyung Kim; Teoan Kim

doi:10.1002/mrd.20860

Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor

Sun Kwon Mo, Chul Koo Bon, Ryul Choi Bok, Yoon Yub Park, Man Lee Young, Suk Suh Hun, Sik Park Young, Taek Lee Hoon, Jin Hoi Kim, Yeol Roh Ji, Nam Hyung Kim, Teoan Kim

Department of Animal Biotechnology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We report here the generation of transgenic chickens that produce human granulocyte-colony stimulating factor (hG-CSF) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector-encoded hG-GSF gene. The biological activity of the recombinant hG-CSF was significantly higher than its commercially derived E. coli-derived counterpart. Successful germline transmission of the transgene was also confirmed in G₁ transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G₁ progeny were dead within 1 month of hatching.

Original language	English
Pages (from-to)	1120-1126
Number of pages	7
Journal	Molecular Reproduction and Development
Volume	75
Issue number	7
DOIs	https://doi.org/10.1002/mrd.20860
State	Published - Jul 2008

Keywords

Human granulocyte-colony stimulating factor (hG-CSF)
Retrovirus vector
Transgenic chicken

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title = "Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor",

abstract = "We report here the generation of transgenic chickens that produce human granulocyte-colony stimulating factor (hG-CSF) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector-encoded hG-GSF gene. The biological activity of the recombinant hG-CSF was significantly higher than its commercially derived E. coli-derived counterpart. Successful germline transmission of the transgene was also confirmed in G1 transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G1 progeny were dead within 1 month of hatching.",

keywords = "Human granulocyte-colony stimulating factor (hG-CSF), Retrovirus vector, Transgenic chicken",

author = "Mo, {Sun Kwon} and Bon, {Chul Koo} and Bok, {Ryul Choi} and Park, {Yoon Yub} and Young, {Man Lee} and Hun, {Suk Suh} and Young, {Sik Park} and Hoon, {Taek Lee} and Kim, {Jin Hoi} and Ji, {Yeol Roh} and Kim, {Nam Hyung} and Teoan Kim",

year = "2008",

month = jul,

doi = "10.1002/mrd.20860",

language = "English",

volume = "75",

pages = "1120--1126",

journal = "Molecular Reproduction and Development",

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AU - Mo, Sun Kwon

AU - Bon, Chul Koo

AU - Bok, Ryul Choi

AU - Park, Yoon Yub

AU - Young, Man Lee

AU - Hun, Suk Suh

AU - Young, Sik Park

AU - Hoon, Taek Lee

AU - Kim, Jin Hoi

AU - Ji, Yeol Roh

AU - Kim, Nam Hyung

AU - Kim, Teoan

PY - 2008/7

Y1 - 2008/7

N2 - We report here the generation of transgenic chickens that produce human granulocyte-colony stimulating factor (hG-CSF) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector-encoded hG-GSF gene. The biological activity of the recombinant hG-CSF was significantly higher than its commercially derived E. coli-derived counterpart. Successful germline transmission of the transgene was also confirmed in G1 transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G1 progeny were dead within 1 month of hatching.

AB - We report here the generation of transgenic chickens that produce human granulocyte-colony stimulating factor (hG-CSF) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector-encoded hG-GSF gene. The biological activity of the recombinant hG-CSF was significantly higher than its commercially derived E. coli-derived counterpart. Successful germline transmission of the transgene was also confirmed in G1 transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G1 progeny were dead within 1 month of hatching.

KW - Human granulocyte-colony stimulating factor (hG-CSF)

KW - Retrovirus vector

KW - Transgenic chicken

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DO - 10.1002/mrd.20860

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VL - 75

SP - 1120

EP - 1126

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

IS - 7

ER -

Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor

Abstract

Keywords

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