Abstract
Group A acetylsaponins are the main causative components for bitter and astringent tastes of soybean (Glycine max). In this study, we examined the genetic nature of the absence of group A acetylsaponins in 12 Korean wild soybean (Glycine soja) accessions. In all 12 accessions, the coding region (1431-bp) of Sg-1 locus was identical with Sg-1a, which adds the xylose sugar moiety at the terminal position of the C-22 sugar chain of SS-A, except one nucleotide (G→A change) at +948th position. This point mutation results in change of one amino acid from tryptophan (TGG) to stop codon (TGA). We observed that the mutated Sg-1 was controlled by a single recessive gene (sg-10-a1). This gene was mapped between BARCSOYSSR_07_1561 and BARCSOYSSR_07_1598 on soybean chromosome 7. Our study demonstrated that the mutated Sg-1 gene in Korean wild soybeans is genetically different from those identified in Japanese soybean cultivar 'Kinusayaka' and wild soybean JP-36121. We believe that the new Sg-1 mutants can also be utilized to produce a new soybean variety without bitter and astringent properties.
Original language | English |
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Pages (from-to) | 316-321 |
Number of pages | 6 |
Journal | Plant Breeding |
Volume | 134 |
Issue number | 3 |
DOIs | |
State | Published - 1 Jun 2015 |
Keywords
- C-22 sugar chain biosynthesis
- Glycine soja
- Group A saponins
- Soyasaponins
- Soybean breeding
- Wild soybean