Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea

Eun Jin Choi, Chang Hee Lee, Jae Young Song, Hee Jong Song, Choi Kyu Park, Byounghan Kim, Yeun Kyung Shin

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms were positive for type 1 PRRSV while 198 farms were positive for type 2. Nucleotide sequences encoding the open reading frame (ORF) 5 were analyzed and compared to those of various published PRRSV isolates obtained worldwide. Sequence identity of the ORF 5 in the isolates was 81.6̃100% for type 1 viruses and 81.4̃100% for type 2 viruses. Phylogenetic analysis of the ORF 5 sequences showed that types 1 and 2 PRRSVs from Korea were mainly classified into three and four clusters, respectively. The analyzed isolates were distributed throughout the clusters independent of the isolation year or geographical origin. In conclusion, our results indicated that the genetic diversity of PRRSVs from Korean pig farms is high and has been increasing over time.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalJournal of Veterinary Science
Volume14
Issue number2
DOIs
StatePublished - 2013

Keywords

  • Korea
  • Open reading frame 5
  • Phylogenetic analysis
  • Porcine reproductive and respiratory syndrome virus

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