Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Shukho Kim; Hyesoon Song; Jong Sook Jin; Weon Ju Lee; Jungmin Kim

doi:10.3390/antibiotics11081041

Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Shukho Kim, Hyesoon Song, Jong Sook Jin, Weon Ju Lee, Jungmin Kim

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT² buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.

Original language	English
Article number	1041
Journal	Antibiotics
Volume	11
Issue number	8
DOIs	https://doi.org/10.3390/antibiotics11081041
State	Published - Aug 2022

Keywords

bacteriophage
Cutibacterium acnes
hydrophobicity
phage therapy

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/antibiotics11081041

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title = "Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients",

abstract = "Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT2 buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.",

keywords = "bacteriophage, Cutibacterium acnes, hydrophobicity, phage therapy",

author = "Shukho Kim and Hyesoon Song and Jin, {Jong Sook} and Lee, {Weon Ju} and Jungmin Kim",

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volume = "11",

journal = "Antibiotics",

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T1 - Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

AU - Kim, Shukho

AU - Song, Hyesoon

AU - Jin, Jong Sook

AU - Lee, Weon Ju

AU - Kim, Jungmin

PY - 2022/8

Y1 - 2022/8

N2 - Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT2 buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.

AB - Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT2 buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.

KW - bacteriophage

KW - Cutibacterium acnes

KW - hydrophobicity

KW - phage therapy

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U2 - 10.3390/antibiotics11081041

DO - 10.3390/antibiotics11081041

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Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Abstract

Keywords

UN SDGs

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