Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea

Sang Yeop Lee; Man Hwan Oh; Sung Ho Yun; Chi Won Choi; Edmond Changkyun Park; Hyun Seok Song; Hayoung Lee; Yoon Sun Yi; Juhyun Shin; Chaeuk Chung; Jae Young Moon; Je Chul Lee; Gun Hwa Kim; Seung Il Kim

doi:10.1016/j.meegid.2018.07.030

Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea

Sang Yeop Lee, Man Hwan Oh, Sung Ho Yun, Chi Won Choi, Edmond Changkyun Park, Hyun Seok Song, Hayoung Lee, Yoon Sun Yi, Juhyun Shin, Chaeuk Chung, Jae Young Moon, Je Chul Lee, Gun Hwa Kim, Seung Il Kim

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Extensively drug-resistant (XDR) Acinetobacter baumannii strains have emerged rapidly worldwide. The antibiotic resistance characteristics of XDR A. baumannii strains show regional differences; therefore, it is necessary to analyze both genomic and proteomic characteristics of emerging XDR A. baumannii clinical strains isolated in Korea to elucidate their multidrug resistance. Here, we isolated new sequence type of XDR A. baumannii clinical strain (KAB03) from Korean hospitals and performed comprehensive genome analyses. The strain belongs to new sequence type, ST451. Single nucleotide polymorphism (SNP) analysis with other types of A. baumannii strains revealed that KAB03 has unique SNP pattern in the regions of gyrB and gpi of MLST profiles. A. baumannii KAB03 harbours three antibiotic resistance islands (AbGRI1, 2, and 3). AbGRI1 harbours two copies of Tn2006 containing bla_OXA-23, which play an important role in antibiotic resistance. AbGRI2 possesses aminoglycoside resistant gene aph(3′)-Ic and class A β-lactamase bla_TEM. AbGIR3 has macrolide resistant genes and aminoglycoside resistant gene armA. A. baumannii KAB03 harbours mutations in pmrB and pmrC, which are believed to confer colistin resistance. In addition, proteomic and transcriptional analysis of KAB03 confirmed that β-lactamases (ADC-73 and OXA-23), Ade efflux pumps (AdeIJK), outer membrane proteins (OmpA and OmpW), and colistin resistance genes (PmrCAB) were major proteins responsible for antibiotic resistance. Our proteogenomic results provide valuable information for multi-drug resistance in emerging XDR A. baumannii strains belonging to ST451.

Original language	English
Pages (from-to)	150-158
Number of pages	9
Journal	Infection, Genetics and Evolution
Volume	65
DOIs	https://doi.org/10.1016/j.meegid.2018.07.030
State	Published - Nov 2018

Keywords

Acinetobacter baumannii of ST451
Antibiotic resistance genes
Comparative genomics
LC-MS based proteomics
SNP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.meegid.2018.07.030

Cite this

Lee, S. Y., Oh, M. H., Yun, S. H., Choi, C. W., Park, E. C., Song, H. S., Lee, H., Yi, Y. S., Shin, J., Chung, C., Moon, J. Y., Lee, J. C., Kim, G. H., & Kim, S. I. (2018). Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea. Infection, Genetics and Evolution, 65, 150-158. https://doi.org/10.1016/j.meegid.2018.07.030

@article{0d43639cf5cd41208ab2ac6aa04d0b43,

title = "Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea",

abstract = "Extensively drug-resistant (XDR) Acinetobacter baumannii strains have emerged rapidly worldwide. The antibiotic resistance characteristics of XDR A. baumannii strains show regional differences; therefore, it is necessary to analyze both genomic and proteomic characteristics of emerging XDR A. baumannii clinical strains isolated in Korea to elucidate their multidrug resistance. Here, we isolated new sequence type of XDR A. baumannii clinical strain (KAB03) from Korean hospitals and performed comprehensive genome analyses. The strain belongs to new sequence type, ST451. Single nucleotide polymorphism (SNP) analysis with other types of A. baumannii strains revealed that KAB03 has unique SNP pattern in the regions of gyrB and gpi of MLST profiles. A. baumannii KAB03 harbours three antibiotic resistance islands (AbGRI1, 2, and 3). AbGRI1 harbours two copies of Tn2006 containing blaOXA-23, which play an important role in antibiotic resistance. AbGRI2 possesses aminoglycoside resistant gene aph(3′)-Ic and class A β-lactamase blaTEM. AbGIR3 has macrolide resistant genes and aminoglycoside resistant gene armA. A. baumannii KAB03 harbours mutations in pmrB and pmrC, which are believed to confer colistin resistance. In addition, proteomic and transcriptional analysis of KAB03 confirmed that β-lactamases (ADC-73 and OXA-23), Ade efflux pumps (AdeIJK), outer membrane proteins (OmpA and OmpW), and colistin resistance genes (PmrCAB) were major proteins responsible for antibiotic resistance. Our proteogenomic results provide valuable information for multi-drug resistance in emerging XDR A. baumannii strains belonging to ST451.",

keywords = "Acinetobacter baumannii of ST451, Antibiotic resistance genes, Comparative genomics, LC-MS based proteomics, SNP",

author = "Lee, {Sang Yeop} and Oh, {Man Hwan} and Yun, {Sung Ho} and Choi, {Chi Won} and Park, {Edmond Changkyun} and Song, {Hyun Seok} and Hayoung Lee and Yi, {Yoon Sun} and Juhyun Shin and Chaeuk Chung and Moon, {Jae Young} and Lee, {Je Chul} and Kim, {Gun Hwa} and Kim, {Seung Il}",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = nov,

doi = "10.1016/j.meegid.2018.07.030",

language = "English",

volume = "65",

pages = "150--158",

journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea

AU - Lee, Sang Yeop

AU - Oh, Man Hwan

AU - Yun, Sung Ho

AU - Choi, Chi Won

AU - Park, Edmond Changkyun

AU - Song, Hyun Seok

AU - Lee, Hayoung

AU - Yi, Yoon Sun

AU - Shin, Juhyun

AU - Chung, Chaeuk

AU - Moon, Jae Young

AU - Lee, Je Chul

AU - Kim, Gun Hwa

AU - Kim, Seung Il

PY - 2018/11

Y1 - 2018/11

N2 - Extensively drug-resistant (XDR) Acinetobacter baumannii strains have emerged rapidly worldwide. The antibiotic resistance characteristics of XDR A. baumannii strains show regional differences; therefore, it is necessary to analyze both genomic and proteomic characteristics of emerging XDR A. baumannii clinical strains isolated in Korea to elucidate their multidrug resistance. Here, we isolated new sequence type of XDR A. baumannii clinical strain (KAB03) from Korean hospitals and performed comprehensive genome analyses. The strain belongs to new sequence type, ST451. Single nucleotide polymorphism (SNP) analysis with other types of A. baumannii strains revealed that KAB03 has unique SNP pattern in the regions of gyrB and gpi of MLST profiles. A. baumannii KAB03 harbours three antibiotic resistance islands (AbGRI1, 2, and 3). AbGRI1 harbours two copies of Tn2006 containing blaOXA-23, which play an important role in antibiotic resistance. AbGRI2 possesses aminoglycoside resistant gene aph(3′)-Ic and class A β-lactamase blaTEM. AbGIR3 has macrolide resistant genes and aminoglycoside resistant gene armA. A. baumannii KAB03 harbours mutations in pmrB and pmrC, which are believed to confer colistin resistance. In addition, proteomic and transcriptional analysis of KAB03 confirmed that β-lactamases (ADC-73 and OXA-23), Ade efflux pumps (AdeIJK), outer membrane proteins (OmpA and OmpW), and colistin resistance genes (PmrCAB) were major proteins responsible for antibiotic resistance. Our proteogenomic results provide valuable information for multi-drug resistance in emerging XDR A. baumannii strains belonging to ST451.

AB - Extensively drug-resistant (XDR) Acinetobacter baumannii strains have emerged rapidly worldwide. The antibiotic resistance characteristics of XDR A. baumannii strains show regional differences; therefore, it is necessary to analyze both genomic and proteomic characteristics of emerging XDR A. baumannii clinical strains isolated in Korea to elucidate their multidrug resistance. Here, we isolated new sequence type of XDR A. baumannii clinical strain (KAB03) from Korean hospitals and performed comprehensive genome analyses. The strain belongs to new sequence type, ST451. Single nucleotide polymorphism (SNP) analysis with other types of A. baumannii strains revealed that KAB03 has unique SNP pattern in the regions of gyrB and gpi of MLST profiles. A. baumannii KAB03 harbours three antibiotic resistance islands (AbGRI1, 2, and 3). AbGRI1 harbours two copies of Tn2006 containing blaOXA-23, which play an important role in antibiotic resistance. AbGRI2 possesses aminoglycoside resistant gene aph(3′)-Ic and class A β-lactamase blaTEM. AbGIR3 has macrolide resistant genes and aminoglycoside resistant gene armA. A. baumannii KAB03 harbours mutations in pmrB and pmrC, which are believed to confer colistin resistance. In addition, proteomic and transcriptional analysis of KAB03 confirmed that β-lactamases (ADC-73 and OXA-23), Ade efflux pumps (AdeIJK), outer membrane proteins (OmpA and OmpW), and colistin resistance genes (PmrCAB) were major proteins responsible for antibiotic resistance. Our proteogenomic results provide valuable information for multi-drug resistance in emerging XDR A. baumannii strains belonging to ST451.

KW - Acinetobacter baumannii of ST451

KW - Antibiotic resistance genes

KW - Comparative genomics

KW - LC-MS based proteomics

KW - SNP

UR - http://www.scopus.com/inward/record.url?scp=85050535717&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2018.07.030

DO - 10.1016/j.meegid.2018.07.030

M3 - Article

C2 - 30053642

AN - SCOPUS:85050535717

SN - 1567-1348

VL - 65

SP - 150

EP - 158

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

ER -

Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this