Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal

Se Hyun Son; Kwang Won Seo; Yeong Bin Kim; Eun Bi Noh; Keun Woo Lee; Tae Ho Oh; Seung Joon Kim; Jae Chan Song; Tae Wan Kim; Young Ju Lee

doi:10.14405/kjvr.2020.60.3.173

Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal

Se Hyun Son, Kwang Won Seo, Yeong Bin Kim, Eun Bi Noh, Keun Woo Lee, Tae Ho Oh, Seung Joon Kim, Jae Chan Song, Tae Wan Kim, Young Ju Lee

Department of Veterinary Medicine

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.

Original language	English
Pages (from-to)	173-177
Number of pages	5
Journal	Korean Journal of Veterinary Research
Volume	60
Issue number	3
DOIs	https://doi.org/10.14405/kjvr.2020.60.3.173
State	Published - 2020

Keywords

Edible offal
Escherichia coli
Fluoroquinolones
Molecular typing
Quinolone resistance-determining region

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.14405/kjvr.2020.60.3.173

Cite this

@article{65d5bff741d2431bb273d8ceec54e200,

title = "Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal",

abstract = "Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.",

keywords = "Edible offal, Escherichia coli, Fluoroquinolones, Molecular typing, Quinolone resistance-determining region",

author = "Son, {Se Hyun} and Seo, {Kwang Won} and Kim, {Yeong Bin} and Noh, {Eun Bi} and Lee, {Keun Woo} and Oh, {Tae Ho} and Kim, {Seung Joon} and Song, {Jae Chan} and Kim, {Tae Wan} and Lee, {Young Ju}",

year = "2020",

doi = "10.14405/kjvr.2020.60.3.173",

language = "English",

volume = "60",

pages = "173--177",

journal = "Korean Journal of Veterinary Research",

issn = "2466-1384",

publisher = "Korean Society of Veterinary Science",

number = "3",

}

TY - JOUR

T1 - Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal

AU - Son, Se Hyun

AU - Seo, Kwang Won

AU - Kim, Yeong Bin

AU - Noh, Eun Bi

AU - Lee, Keun Woo

AU - Oh, Tae Ho

AU - Kim, Seung Joon

AU - Song, Jae Chan

AU - Kim, Tae Wan

AU - Lee, Young Ju

PY - 2020

Y1 - 2020

N2 - Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.

AB - Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.

KW - Edible offal

KW - Escherichia coli

KW - Fluoroquinolones

KW - Molecular typing

KW - Quinolone resistance-determining region

UR - http://www.scopus.com/inward/record.url?scp=85092706802&partnerID=8YFLogxK

U2 - 10.14405/kjvr.2020.60.3.173

DO - 10.14405/kjvr.2020.60.3.173

M3 - Article

AN - SCOPUS:85092706802

SN - 2466-1384

VL - 60

SP - 173

EP - 177

JO - Korean Journal of Veterinary Research

JF - Korean Journal of Veterinary Research

IS - 3

ER -

Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this