Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences

T. S. Yong; S. J. Park; U. W. Hwang; H. W. Yang; K. W. Lee; D. Y. Min; H. J. Rim; Y. Wang; F. Zheng

doi:10.1645/0022-3395(2000)086[0887:goglif]2.0.co;2

Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences

T. S. Yong, S. J. Park, U. W. Hwang, H. W. Yang, K. W. Lee, D. Y. Min, H. J. Rim, Y. Wang, F. Zheng

Department of Biology Education

Yonsei University

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.

Original language	English
Pages (from-to)	887-891
Number of pages	5
Journal	Journal of Parasitology
Volume	86
Issue number	4
DOIs	https://doi.org/10.1645/0022-3395(2000)086[0887:goglif]2.0.co;2
State	Published - 2000

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@article{0ba61871f0864e89b075417d8891fb15,

title = "Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences",

abstract = "Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.",

author = "Yong, {T. S.} and Park, {S. J.} and Hwang, {U. W.} and Yang, {H. W.} and Lee, {K. W.} and Min, {D. Y.} and Rim, {H. J.} and Y. Wang and F. Zheng",

year = "2000",

doi = "10.1645/0022-3395(2000)086[0887:goglif]2.0.co;2",

language = "English",

volume = "86",

pages = "887--891",

journal = "Journal of Parasitology",

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TY - JOUR

T1 - Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences

AU - Yong, T. S.

AU - Park, S. J.

AU - Hwang, U. W.

AU - Yang, H. W.

AU - Lee, K. W.

AU - Min, D. Y.

AU - Rim, H. J.

AU - Wang, Y.

AU - Zheng, F.

PY - 2000

Y1 - 2000

N2 - Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.

AB - Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.

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DO - 10.1645/0022-3395(2000)086[0887:goglif]2.0.co;2

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VL - 86

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EP - 891

JO - Journal of Parasitology

JF - Journal of Parasitology

IS - 4

ER -

Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences

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