TY - JOUR
T1 - Ginsenosides from the fruits of Panax ginseng and their cytotoxic effects on human cancer cell lines
AU - Gwag, Jung Eun
AU - Lee, Yeong Geun
AU - Hwang-Bo, Jeon
AU - Kim, Hyoung Geun
AU - Oh, Seon Min
AU - Lee, Dae Young
AU - Baek, Nam In
N1 - Publisher Copyright:
© The Korean Society for Applied Biological Chemistry 2018.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The fruits of Panax ginseng were extracted with 80% aqueous MeOH and the concentrates were partitioned into EtOAc, n-BuOH, and H2O fractions. The repeated SiO2 and octadecyl SiO2 column chromatographies for the EtOAc fraction led to isolation of five ginsenosides. The chemical structures of these compounds were determined as ginsenoside F1 (1), ginsenoside F2 (2), ginsenoside F3 (3), ginsenoside Ia (4), notoginsenoside Fe (5) based on spectroscopic analyses including nuclear magnetic resonance, MS, and infrared. Compounds 2-5 were isolated for the first time from the fruits of P. ginseng in this study. All isolated compounds were evaluated for cytotoxic activities against human cancer cell lines such as HCT-116, SK-OV-3, human cervix adenocarcinoma (HeLa), HepG2, and SK-MEL-5. Among them compounds 2, 4, and 5 showed significant cytotoxicity on cancer cells. Compound 2 exhibited cytotoxicity on SK-MEL-5, HepG2, and HeLa cells with IC50 values of 82.8, 86.8, and 78.3 µM, respectively. Compound 4 showed cytotoxicity on HCT-116, SK-MEL-5, SK-OV-3, HepG2, and HeLa cells with IC50 values of 24.5, 25.4, 26.3, 22.0, and 24.9 µM, respectively. Compound 5 did on SK-MEL-5 cell with IC50 value of 81.7 µM. The cytotoxicity of ginsenoside 2, 4, and 5 isolated from the fruits of Panax ginseng showed strong inhibition effect against on cancer cells, all of which have a glucopyranosyl moiety on C-3.
AB - The fruits of Panax ginseng were extracted with 80% aqueous MeOH and the concentrates were partitioned into EtOAc, n-BuOH, and H2O fractions. The repeated SiO2 and octadecyl SiO2 column chromatographies for the EtOAc fraction led to isolation of five ginsenosides. The chemical structures of these compounds were determined as ginsenoside F1 (1), ginsenoside F2 (2), ginsenoside F3 (3), ginsenoside Ia (4), notoginsenoside Fe (5) based on spectroscopic analyses including nuclear magnetic resonance, MS, and infrared. Compounds 2-5 were isolated for the first time from the fruits of P. ginseng in this study. All isolated compounds were evaluated for cytotoxic activities against human cancer cell lines such as HCT-116, SK-OV-3, human cervix adenocarcinoma (HeLa), HepG2, and SK-MEL-5. Among them compounds 2, 4, and 5 showed significant cytotoxicity on cancer cells. Compound 2 exhibited cytotoxicity on SK-MEL-5, HepG2, and HeLa cells with IC50 values of 82.8, 86.8, and 78.3 µM, respectively. Compound 4 showed cytotoxicity on HCT-116, SK-MEL-5, SK-OV-3, HepG2, and HeLa cells with IC50 values of 24.5, 25.4, 26.3, 22.0, and 24.9 µM, respectively. Compound 5 did on SK-MEL-5 cell with IC50 value of 81.7 µM. The cytotoxicity of ginsenoside 2, 4, and 5 isolated from the fruits of Panax ginseng showed strong inhibition effect against on cancer cells, all of which have a glucopyranosyl moiety on C-3.
KW - Cytotoxicity
KW - Fruit
KW - Ginsenoside
KW - Human cancer cells
KW - Panax ginseng
UR - http://www.scopus.com/inward/record.url?scp=85060841015&partnerID=8YFLogxK
U2 - 10.3839/jabc.2018.052
DO - 10.3839/jabc.2018.052
M3 - Article
AN - SCOPUS:85060841015
SN - 1976-0442
VL - 61
SP - 371
EP - 377
JO - Journal of Applied Biological Chemistry
JF - Journal of Applied Biological Chemistry
IS - 4
ER -
