Global histidine phosphoproteomics in human prostate cancer cells

Yan Gao; Doeun Kim; Eunji Sung; Minjia Tan; Tae Gyun Kwon; Jun Nyung Lee; Sangkyu Lee

doi:10.5478/MSL.2020.11.3.52

Global histidine phosphoproteomics in human prostate cancer cells

Yan Gao, Doeun Kim, Eunji Sung, Minjia Tan, Tae Gyun Kwon, Jun Nyung Lee, Sangkyu Lee

Department of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Histidine phosphorylation (pHis) is increasingly recognized as an important post translational modification (PTM) in regulating cellular functions in eukaryotes. In order to clarify the role of pHis in mammalian cell signaling system, a global phos-phorylation study was performed in human prostate cancer cells, PC-3M, using a TiO₂ affinity chromatography. A total number of 307 pHis sites were identified on the 268 proteins among total identified 9,924 phosphorylation sites on 3,316 proteins. In addition, 22 pHis proteins were classified in enzyme category. This report provides the first database for the study of pHis in prostate cancer cells.

Original language	English
Pages (from-to)	52-58
Number of pages	7
Journal	Mass Spectrometry Letters
Volume	11
Issue number	3
DOIs	https://doi.org/10.5478/MSL.2020.11.3.52
State	Published - 2020

Keywords

Histidine phosphorylation
LC-MS
Mammalian cells
TiO-affinity chromatography

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.5478/MSL.2020.11.3.52

Cite this

@article{e38a7a6614f04606832145e23cfa0a7c,

title = "Global histidine phosphoproteomics in human prostate cancer cells",

abstract = "Histidine phosphorylation (pHis) is increasingly recognized as an important post translational modification (PTM) in regulating cellular functions in eukaryotes. In order to clarify the role of pHis in mammalian cell signaling system, a global phos-phorylation study was performed in human prostate cancer cells, PC-3M, using a TiO2 affinity chromatography. A total number of 307 pHis sites were identified on the 268 proteins among total identified 9,924 phosphorylation sites on 3,316 proteins. In addition, 22 pHis proteins were classified in enzyme category. This report provides the first database for the study of pHis in prostate cancer cells.",

keywords = "Histidine phosphorylation, LC-MS, Mammalian cells, TiO-affinity chromatography",

author = "Yan Gao and Doeun Kim and Eunji Sung and Minjia Tan and Kwon, {Tae Gyun} and Lee, {Jun Nyung} and Sangkyu Lee",

note = "Publisher Copyright: {\textcopyright} Korean Society for Mass Spectrometry.",

year = "2020",

doi = "10.5478/MSL.2020.11.3.52",

language = "English",

volume = "11",

pages = "52--58",

journal = "Mass Spectrometry Letters",

issn = "2233-4203",

publisher = "Korean Society Mass Spectrometry",

number = "3",

}

TY - JOUR

T1 - Global histidine phosphoproteomics in human prostate cancer cells

AU - Gao, Yan

AU - Kim, Doeun

AU - Sung, Eunji

AU - Tan, Minjia

AU - Kwon, Tae Gyun

AU - Lee, Jun Nyung

AU - Lee, Sangkyu

N1 - Publisher Copyright: © Korean Society for Mass Spectrometry.

PY - 2020

Y1 - 2020

N2 - Histidine phosphorylation (pHis) is increasingly recognized as an important post translational modification (PTM) in regulating cellular functions in eukaryotes. In order to clarify the role of pHis in mammalian cell signaling system, a global phos-phorylation study was performed in human prostate cancer cells, PC-3M, using a TiO2 affinity chromatography. A total number of 307 pHis sites were identified on the 268 proteins among total identified 9,924 phosphorylation sites on 3,316 proteins. In addition, 22 pHis proteins were classified in enzyme category. This report provides the first database for the study of pHis in prostate cancer cells.

AB - Histidine phosphorylation (pHis) is increasingly recognized as an important post translational modification (PTM) in regulating cellular functions in eukaryotes. In order to clarify the role of pHis in mammalian cell signaling system, a global phos-phorylation study was performed in human prostate cancer cells, PC-3M, using a TiO2 affinity chromatography. A total number of 307 pHis sites were identified on the 268 proteins among total identified 9,924 phosphorylation sites on 3,316 proteins. In addition, 22 pHis proteins were classified in enzyme category. This report provides the first database for the study of pHis in prostate cancer cells.

KW - Histidine phosphorylation

KW - LC-MS

KW - Mammalian cells

KW - TiO-affinity chromatography

UR - http://www.scopus.com/inward/record.url?scp=85095760024&partnerID=8YFLogxK

U2 - 10.5478/MSL.2020.11.3.52

DO - 10.5478/MSL.2020.11.3.52

M3 - Article

AN - SCOPUS:85095760024

SN - 2233-4203

VL - 11

SP - 52

EP - 58

JO - Mass Spectrometry Letters

JF - Mass Spectrometry Letters

IS - 3

ER -

Global histidine phosphoproteomics in human prostate cancer cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this