Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface

Mashooq Khan, Soo Young Park

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Glucose oxidase (GOx) and horseradish peroxidase (HRP) were co-immobilized to the polyacrylicacid block of a poly(acrylicacid-. b-4-cyanobiphenyl-4'-undecylacrylate) (PAA-. b-LCP) copolymer in water. PAA-. b-LCP was strongly anchored by the LCP block in 4-cyano-4'-pentylbiphenyl (5CB) which was contained in a transmission electron microscope (TEM) grid for glucose detection. The optimal conditions for the performance of the TEM grid glucose biosensor were studied in terms of the activity and stability of the immobilized enzymes. Glucose in water was detected by the 5CB changing from a planar to a homeotropic orientation, as observed through a polarized optical microscope. The TEM biosensor detected glucose concentrations at ≥0.02. mM, with an optimal GOx/HRP molar ratio of 3/1. This glucose biosensor has characteristics of enzyme sensitivity and stability, reusability, the ease and selective glucose detection which may provide a new way of detecting glucose.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalJournal of Colloid and Interface Science
Volume457
DOIs
StatePublished - 1 Nov 2015

Keywords

  • Bienzyme
  • Biosensor
  • Glucose
  • Glucose oxidase
  • Liquid crystal
  • Peroxidase

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