Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 Cells in vitro and S180 cells in vivo

Mohammad Lalmoddin Mollah; Jung Ok Kim; Gee Dong Lee; Chang Ho Park; Joo Heon Hong; Hak Yoon Kim; Kil Soo Kim

doi:10.1080/08923970902810432

Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 Cells in vitro and S180 cells in vivo

Mohammad Lalmoddin Mollah, Jung Ok Kim, Gee Dong Lee, Chang Ho Park, Joo Heon Hong, Hak Yoon Kim, Kil Soo Kim

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The effects of Bulnesia sarmienti (BS) aqueous extract on the cell growth of A549 cell lines were investigated. BS has strong cytotoxic activity on the A549 cell lines (IC50; less than 100μg/mL) in MTT assay. HPLC confirmed that BS contains catechins as major compound. Cell cycle analysis by flow cytometry indicated that BS arrested the cell cycle in the sub-G1 phase. BS induced DNA fragmentation, and increased the expression of the p53 protein in immunoblot analysis. These results indicated that the anticancer effect of BS was mediated via the process of apoptosis and growth-inhibition.

Original language	English
Pages (from-to)	492-498
Number of pages	7
Journal	Immunopharmacology and Immunotoxicology
Volume	31
Issue number	3
DOIs	https://doi.org/10.1080/08923970902810432
State	Published - 2009

Keywords

A549 cells
Apoptosis
Bulnesia sarmienti
Cytotoxicity
Growth inhibition

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10.1080/08923970902810432

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title = "Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 Cells in vitro and S180 cells in vivo",

abstract = "The effects of Bulnesia sarmienti (BS) aqueous extract on the cell growth of A549 cell lines were investigated. BS has strong cytotoxic activity on the A549 cell lines (IC50; less than 100μg/mL) in MTT assay. HPLC confirmed that BS contains catechins as major compound. Cell cycle analysis by flow cytometry indicated that BS arrested the cell cycle in the sub-G1 phase. BS induced DNA fragmentation, and increased the expression of the p53 protein in immunoblot analysis. These results indicated that the anticancer effect of BS was mediated via the process of apoptosis and growth-inhibition.",

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author = "Mollah, {Mohammad Lalmoddin} and Kim, {Jung Ok} and Lee, {Gee Dong} and Park, {Chang Ho} and Hong, {Joo Heon} and Kim, {Hak Yoon} and Kim, {Kil Soo}",

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AU - Mollah, Mohammad Lalmoddin

AU - Kim, Jung Ok

AU - Lee, Gee Dong

AU - Park, Chang Ho

AU - Hong, Joo Heon

AU - Kim, Hak Yoon

AU - Kim, Kil Soo

PY - 2009

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AB - The effects of Bulnesia sarmienti (BS) aqueous extract on the cell growth of A549 cell lines were investigated. BS has strong cytotoxic activity on the A549 cell lines (IC50; less than 100μg/mL) in MTT assay. HPLC confirmed that BS contains catechins as major compound. Cell cycle analysis by flow cytometry indicated that BS arrested the cell cycle in the sub-G1 phase. BS induced DNA fragmentation, and increased the expression of the p53 protein in immunoblot analysis. These results indicated that the anticancer effect of BS was mediated via the process of apoptosis and growth-inhibition.

KW - A549 cells

KW - Apoptosis

KW - Bulnesia sarmienti

KW - Cytotoxicity

KW - Growth inhibition

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ER -

Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 Cells in vitro and S180 cells in vivo

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