H3K9 histone methyltransferase G9a-mediated transcriptional activation of p21

Si Taek Oh; Kee Beom Kim; Yun Cheol Chae; Joo Young Kang; Yoonsoo Hahn; Sang Beom Seo

doi:10.1016/j.febslet.2014.01.039

H3K9 histone methyltransferase G9a-mediated transcriptional activation of p21

Si Taek Oh, Kee Beom Kim, Yun Cheol Chae, Joo Young Kang, Yoonsoo Hahn, Sang Beom Seo

Chung-Ang University

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21, in p53-null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with PCAF, and provide evidence that the activating complex is recruited to the p21 promoter upon DNA damage-inducing agent etoposide treatment. Our study suggests that G9a decreases proliferation and cell viability by increasing the level of p21-mediated apoptosis. Our results suggest that G9a functions as a coactivator for p21 transcription, and directs cells to undergo apoptosis.

Original language	English
Pages (from-to)	685-691
Number of pages	7
Journal	FEBS Letters
Volume	588
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2014.01.039
State	Published - 3 Mar 2014

Keywords

Apoptosis
Etoposide
G9a
p21
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10.1016/j.febslet.2014.01.039

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title = "H3K9 histone methyltransferase G9a-mediated transcriptional activation of p21",

abstract = "We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21, in p53-null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with PCAF, and provide evidence that the activating complex is recruited to the p21 promoter upon DNA damage-inducing agent etoposide treatment. Our study suggests that G9a decreases proliferation and cell viability by increasing the level of p21-mediated apoptosis. Our results suggest that G9a functions as a coactivator for p21 transcription, and directs cells to undergo apoptosis.",

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AU - Oh, Si Taek

AU - Kim, Kee Beom

AU - Chae, Yun Cheol

AU - Kang, Joo Young

AU - Hahn, Yoonsoo

AU - Seo, Sang Beom

PY - 2014/3/3

Y1 - 2014/3/3

N2 - We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21, in p53-null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with PCAF, and provide evidence that the activating complex is recruited to the p21 promoter upon DNA damage-inducing agent etoposide treatment. Our study suggests that G9a decreases proliferation and cell viability by increasing the level of p21-mediated apoptosis. Our results suggest that G9a functions as a coactivator for p21 transcription, and directs cells to undergo apoptosis.

AB - We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21, in p53-null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with PCAF, and provide evidence that the activating complex is recruited to the p21 promoter upon DNA damage-inducing agent etoposide treatment. Our study suggests that G9a decreases proliferation and cell viability by increasing the level of p21-mediated apoptosis. Our results suggest that G9a functions as a coactivator for p21 transcription, and directs cells to undergo apoptosis.

KW - Apoptosis

KW - Etoposide

KW - G9a

KW - p21

KW - Transcription

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H3K9 histone methyltransferase G9a-mediated transcriptional activation of p21

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Keywords

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