Helicase translocation assay method using avidin and biotinylated nucleotides

Jae Ho Shin

doi:10.1007/s10529-008-9781-7

Helicase translocation assay method using avidin and biotinylated nucleotides

Jae Ho Shin

School of Applied Bioscience

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Helicase involves many cellular processes that separate double-stranded nucleic acid into single strands. Although it is believed that helicase translocates nucleic acids, it is difficult to show the direct evidence of translocation on nucleic acids. In this study, an avidin-biotinylated nucleotides-based method for helicase translocation assay has been described, and the biochemical assay results have been demonstrated.

Original language	English
Pages (from-to)	2007-2012
Number of pages	6
Journal	Biotechnology Letters
Volume	30
Issue number	11
DOIs	https://doi.org/10.1007/s10529-008-9781-7
State	Published - Nov 2008

Keywords

Avidin
Biotin
DNA translocation assay
Helicase
Translocase

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10.1007/s10529-008-9781-7

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abstract = "Helicase involves many cellular processes that separate double-stranded nucleic acid into single strands. Although it is believed that helicase translocates nucleic acids, it is difficult to show the direct evidence of translocation on nucleic acids. In this study, an avidin-biotinylated nucleotides-based method for helicase translocation assay has been described, and the biochemical assay results have been demonstrated.",

keywords = "Avidin, Biotin, DNA translocation assay, Helicase, Translocase",

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AU - Shin, Jae Ho

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AB - Helicase involves many cellular processes that separate double-stranded nucleic acid into single strands. Although it is believed that helicase translocates nucleic acids, it is difficult to show the direct evidence of translocation on nucleic acids. In this study, an avidin-biotinylated nucleotides-based method for helicase translocation assay has been described, and the biochemical assay results have been demonstrated.

KW - Avidin

KW - Biotin

KW - DNA translocation assay

KW - Helicase

KW - Translocase

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DO - 10.1007/s10529-008-9781-7

M3 - Article

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VL - 30

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EP - 2012

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 11

ER -

Helicase translocation assay method using avidin and biotinylated nucleotides

Abstract

Keywords

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