High-density linkage map reveals QTL for Type-I seed coat cracking in RIL population of soybean [Glycine max (L.) Merr.]

Beom Kyu Kang, Jeong Hyun Seo, Hyun Jo, Krishnanand P. Kulkarni, Man Soo Choi, Hyun Tae Kim, Jeong Dong Lee, Sanjeev K. Dhungana, Hong Sik Kim, Jae Hyeon Oh, Ji Hee Park, Sang Ouk Shin, In Youl Baek

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3 Scopus citations

Abstract

Seed coat cracking (SCC), particularly the Type-I irregular cracking, is critical in determining the quality of appearance and commercial value of soybean seeds. The objective of this study was to identify the quantitative trait loci (QTLs) for SCC with high-density genetic map. One hundred sixty-seven recombinant inbred lines (RILs) developed from a cross between Uram (SCC-resistant) and Chamol (SCC-susceptible) were evaluated for SCC over 2 years (2016–2017). The QTL analysis identified 12 QTLs located on chromosomes 2 (D1b), 6 (C2), 8 (A2), 9 (K), 10 (O), 12 (H), 19 (L), and 20 (I). Out of the 12 QTLs, qSC2-1, qSC9, SC10-1, qSC10-2, and qSC12 were novel QTLs and the other seven QTLs (qSC2-2, qSC2-3, qSC6, qSC8, qSC19-1, qSC19-2, and qSC20) were found to co-localize with the previously identified QTLs. The mean SCC of the RILs of early maturity group was significantly higher than that of the late maturity group, suggesting an association between SCC and maturity loci. In addition, although 10 QTLs were distantly located from the maturity loci (E1, E3, E4, E7, and E10), qSC10-1 and qSC10-2 co-localized with the maturity loci E2. The results obtained in this study provide useful genetic information on SCC which could be used in the SCC breeding programs.

Original languageEnglish
Article number153
JournalEuphytica
Volume216
Issue number10
DOIs
StatePublished - 1 Oct 2020

Keywords

  • Maturity
  • QTL
  • Seed appearance
  • Seed coat cracking
  • Soybean
  • Type-I irregular cracking

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