Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis

Gwang Hyeon Eom; Kabsun Kim; Sung Mi Kim; Hae Jin Kee; Ji Young Kim; Hye Mi Jin; Ju Ryoung Kim; Jung Ha Kim; Nakwon Choe; Kee Beom Kim; Junwon Lee; Hyun Kook; Nacksung Kim; Sang Beom Seo

doi:10.1016/j.bbrc.2009.07.134

Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis

Gwang Hyeon Eom, Kabsun Kim, Sung Mi Kim, Hae Jin Kee, Ji Young Kim, Hye Mi Jin, Ju Ryoung Kim, Jung Ha Kim, Nakwon Choe, Kee Beom Kim, Junwon Lee, Hyun Kook, Nacksung Kim, Sang Beom Seo

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63 Scopus citations

Abstract

A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.

Original language	English
Pages (from-to)	131-136
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	388
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.07.134
State	Published - 9 Oct 2009

Keywords

Histone
Methyltransferase
PRDM8
Steroidogenesis
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10.1016/j.bbrc.2009.07.134

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title = "Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis",

abstract = "A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.",

keywords = "Histone, Methyltransferase, PRDM8, Steroidogenesis, Transcription",

author = "Eom, {Gwang Hyeon} and Kabsun Kim and Kim, {Sung Mi} and Kee, {Hae Jin} and Kim, {Ji Young} and Jin, {Hye Mi} and Kim, {Ju Ryoung} and Kim, {Jung Ha} and Nakwon Choe and Kim, {Kee Beom} and Junwon Lee and Hyun Kook and Nacksung Kim and Seo, {Sang Beom}",

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language = "English",

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AU - Eom, Gwang Hyeon

AU - Kim, Kabsun

AU - Kim, Sung Mi

AU - Kee, Hae Jin

AU - Kim, Ji Young

AU - Jin, Hye Mi

AU - Kim, Ju Ryoung

AU - Kim, Jung Ha

AU - Choe, Nakwon

AU - Kim, Kee Beom

AU - Lee, Junwon

AU - Kook, Hyun

AU - Kim, Nacksung

AU - Seo, Sang Beom

PY - 2009/10/9

Y1 - 2009/10/9

N2 - A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.

AB - A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development.

KW - Histone

KW - Methyltransferase

KW - PRDM8

KW - Steroidogenesis

KW - Transcription

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DO - 10.1016/j.bbrc.2009.07.134

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EP - 136

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis

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