hnRNP Q and PTB modulate the circadian oscillation of mouse Rev-erb α via IRES-mediated translation

Do Yeon Kim, Kyung Chul Woo, Kyung Ha Lee, Tae Don Kim, Kyong Tai Kim

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The physiological and behavioral circadian rhythms of most creatures are controlled by a harmony of functional relationships between clock genes. In mammals, several core clock genes show rhythmic profiles of their mRNA and protein expression. Among them, Rev-erb α functions as a transcriptional repressor, affecting expression patterns of other clock genes. For the continuous and robust oscillation of the molecular clock system, the levels of Reverb α protein are expected to be tightly regulated with the correct timing. Here, we demonstrate that Rev-erb α has an internal ribosomal entry site (IRES) in its 5′ untranslated region. Furthermore, we demonstrate that heterogeneous nuclear ribonucleoprotein Q and polypyrimidine tract-binding protein (PTB) modulate the IRES-mediated translation of Rev-erb α. We suggest that the rhythmic binding affinity of hnRNP Q to the Rev-erb α IRES and the change in PTB cytosolic levels lead to maintenance of the oscillation profile of the Rev-erb α protein.

Original languageEnglish
Pages (from-to)7068-7078
Number of pages11
JournalNucleic Acids Research
Volume38
Issue number20
DOIs
StatePublished - Nov 2010

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