HnRNP q has a suppressive role in the translation of mouse cryptochrome1

Ilgye Lim; Youngseob Jung; Do Yeon Kim; Kyong Tai Kim

doi:10.1371/journal.pone.0159018

HnRNP q has a suppressive role in the translation of mouse cryptochrome1

Ilgye Lim, Youngseob Jung, Do Yeon Kim, Kyong Tai Kim

Department of Dentistry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Precise regulation of gene expression is especially important for circadian timekeeping which is maintained by the proper oscillation of the mRNA and protein of clock genes and clock-controlled genes. As a main component of the core negative arm feedback loops in the circadian clock, the Cry1 gene contributes to the maintenance of behavioral and molecular rhythmicity. Despite the central role of Cry1, the molecular mechanisms regulating expression levels of Cry1 mRNA and protein are not well defined. In particular, the posttranscriptional regulation of Cry1 mRNA fate decisions is unclear. Here, we demonstrate that hnRNP Q binds to mCry1 mRNA via the 50 UTR. Furthermore, hnRNP Q inhibits the translation of mCry1 mRNA, leading to altered rhythmicity in the mCRY1 protein profile.

Original language	English
Article number	e0159018
Journal	PLoS ONE
Volume	11
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0159018
State	Published - Jul 2016

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abstract = "Precise regulation of gene expression is especially important for circadian timekeeping which is maintained by the proper oscillation of the mRNA and protein of clock genes and clock-controlled genes. As a main component of the core negative arm feedback loops in the circadian clock, the Cry1 gene contributes to the maintenance of behavioral and molecular rhythmicity. Despite the central role of Cry1, the molecular mechanisms regulating expression levels of Cry1 mRNA and protein are not well defined. In particular, the posttranscriptional regulation of Cry1 mRNA fate decisions is unclear. Here, we demonstrate that hnRNP Q binds to mCry1 mRNA via the 50 UTR. Furthermore, hnRNP Q inhibits the translation of mCry1 mRNA, leading to altered rhythmicity in the mCRY1 protein profile.",

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HnRNP q has a suppressive role in the translation of mouse cryptochrome1

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