Horseradish Peroxidase Catalyzed Silk-Prefoldin Composite Hydrogel Networks

Jugal Kishore Sahoo, Dawei Xu, Thomas Falcucci, Jaewon Choi, Onur Hasturk, Douglas S. Clark, David L. Kaplan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Protein-based hydrogel biomaterials provide a platform for different biological applications, including the encapsulation and stabilization of different biomolecules. These hydrogel properties can be modulated by controlling the design parameters to match specific needs; thus, multicomponent hydrogels have distinct advantages over single-component hydrogels due to their enhanced versatility. Here, silk fibroin and γ-prefoldin chaperone protein based composite hydrogels were prepared and studied. Different ratios of the proteins were chosen, and the hydrogels were prepared by enzyme-assisted cross-linking. The secondary structure of the two proteins, dityrosine bond formation, and mechanical properties were assessed. The results obtained can be used as a platform for the rational design of composite thermostable hydrogel biomaterials to facilitate protection (due to hydrogel mechanics) and retention of bioactivity (e.g., of enzymes and other biomolecules) due to chaperone-like properties of γ-prefoldin.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalACS Applied Bio Materials
Volume6
Issue number1
DOIs
StatePublished - 16 Jan 2023

Keywords

  • biomaterials
  • dityrosines
  • hydrogels
  • prefoldin
  • proteins
  • silk

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