TY - JOUR
T1 - Hymenobacter terrae sp. nov., a Bacterium Isolated from Soil
AU - Srinivasan, Sathiyaraj
AU - Lee, Jae Jin
AU - Park, Kyoung Ryun
AU - Park, Se Hee
AU - Jung, Hee Young
AU - Kim, Myung Kyum
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media New York.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - A Gram-negative, UV tolerant bacterial strain, DG7AT, was isolated from soil samples collected in Seoul city, South Korea. The cells were grown on R2A agar at 25 °C and were pink to red in color. The DNA G+C content of the novel strain DG7A was 63.5 mol%. Chemotaxonomic data revealed that the strains contain the major fatty acids iso-C15:0, anteiso-C15:0, and summed feature 3 (16:1 ω7c/16:1 ω6c), with phosphatidylethanolamine as the major polar lipid. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain DG7AT formed a distinct phylogenetic line along with Hymenobacter soli PB17T, and they shared approximately 98.35 % 16S rRNA gene sequence similarity. However, these two strains shared only 5.3 % pairwise similarity (reciprocal analysis, 36.3 %) in their genomic DNA. The next highest degree of 16S rRNA gene sequence similarity after H. soli PB17T was found with H. glaciei VUG-A130T (96.78 %), H. antarcticus VUG-A42aaT (96.66 %), and H. saemangeumensis GSR0100T (96.57 %). Based on the phylogenetic analysis and analysis of the physiological and biochemical characteristics, this isolate was considered to represent a novel species, for which we propose the name Hymenobacter terrae sp. nov., with type strain DG7AT (= KCTC 32554T = KEMB 9004-164T= JCM 30007T).
AB - A Gram-negative, UV tolerant bacterial strain, DG7AT, was isolated from soil samples collected in Seoul city, South Korea. The cells were grown on R2A agar at 25 °C and were pink to red in color. The DNA G+C content of the novel strain DG7A was 63.5 mol%. Chemotaxonomic data revealed that the strains contain the major fatty acids iso-C15:0, anteiso-C15:0, and summed feature 3 (16:1 ω7c/16:1 ω6c), with phosphatidylethanolamine as the major polar lipid. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain DG7AT formed a distinct phylogenetic line along with Hymenobacter soli PB17T, and they shared approximately 98.35 % 16S rRNA gene sequence similarity. However, these two strains shared only 5.3 % pairwise similarity (reciprocal analysis, 36.3 %) in their genomic DNA. The next highest degree of 16S rRNA gene sequence similarity after H. soli PB17T was found with H. glaciei VUG-A130T (96.78 %), H. antarcticus VUG-A42aaT (96.66 %), and H. saemangeumensis GSR0100T (96.57 %). Based on the phylogenetic analysis and analysis of the physiological and biochemical characteristics, this isolate was considered to represent a novel species, for which we propose the name Hymenobacter terrae sp. nov., with type strain DG7AT (= KCTC 32554T = KEMB 9004-164T= JCM 30007T).
UR - http://www.scopus.com/inward/record.url?scp=84939975980&partnerID=8YFLogxK
U2 - 10.1007/s00284-014-0760-z
DO - 10.1007/s00284-014-0760-z
M3 - Article
C2 - 25572492
AN - SCOPUS:84939975980
SN - 0343-8651
VL - 70
SP - 643
EP - 650
JO - Current Microbiology
JF - Current Microbiology
IS - 5
ER -