Abstract
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation-specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.
| Original language | English |
|---|---|
| Pages (from-to) | 756-761 |
| Number of pages | 6 |
| Journal | Journal of Korean Medical Science |
| Volume | 16 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2001 |
Keywords
- E-cadherin
- Genitourinary cancer cell lines
- GSTP1
- HMLH1
- Hypermethylation
- Methylation-specific PCR
- MGMT
- Urogenital Neoplasms
- VHL
