Identification and evaluation of flavone-glucosides isolated from barley sprouts and their inhibitory activity against bacterial neuraminidase

Mi Jin Park, Ji Eun Ra, Kyung Hye Seo, Ki Chang Jang, Sang Ik Han, Jin Hwan Lee, Young Hwa Kang, Min Hee Nam, Woo Duck Seo

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Neuraminidase (NA) is one of the key enzymes responsible for bacterial infection and pathogenesis. This study aimed to gain deeper insights into the inhibitory effects of flavone-glucosides (1-9) isolated from barley sprouts (BS) on neuraminidase activity. The isolated compounds were identified as, lutonarin (1), saponarin (2), isoorientin (3), orientin (4), isovitexin (5), isoscoparin-7-O-[6-sinapoyl]-glucoside (6), isoscoparin-7-O-[6-feruloyl]-glucoside (7), isovitexin-7-O-[6-sinapoyl]-glucoside (8), and isovitexin-7-O-[6-feruloyl]-glucoside (9). Among them, compounds 1-5 exhibited neuraminidase-inhibitory activities in a dose-dependent manner, with IC50 values ranging from 20.1 to 32.7 ìM, in a non-competitive inhibition mode according to kinetic studies. Moreover, the individual flavone-glucoside levels differed notably, in particular, lutonarin (1) and saponarin (2) were shown to be present in the greatest amounts, according to UPLC analysis. Consequently, our results suggest that BS may be utilized as an effective NA inhibitor in human health food, additives, and feed.

Original languageEnglish
Pages (from-to)1469-1472
Number of pages4
JournalNatural Product Communications
Volume9
Issue number10
DOIs
StatePublished - Oct 2014

Keywords

  • Barley sprout
  • Enzyme kinetics
  • Flavone-glucosides
  • Neuraminidase

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