Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast

Mohammad Ubaidillah; Kyung A. Kim; Yoon Ha Kim; In Jung Lee; Byung Wook Yun; Doh Hoon Kim; Gary J. Loake; Kyung Min Kim

doi:10.1007/s11033-013-2723-z

Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast

Mohammad Ubaidillah
, Kyung A. Kim
, Yoon Ha Kim
, In Jung Lee
, Byung Wook Yun
, Doh Hoon Kim
, Gary J. Loake
, Kyung Min Kim

Kyungpook National University
Dong-A University
University of Edinburgh

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We identified rice genes that might be involved in drought stress tolerance by virtue of their anti-apoptotic activity. Potential anti-apoptosis related genes were identified by screening an Oryza sativa cDNA library derived from drought stressed tissues in a yeast functional assay. About 28 O. sativa cDNAs promoted yeast survival following engagement of Bax-induced apoptosis. An O. sativa cDNA encoding R12H780 was a highly conserved putative senescence-associated-protein (OsSAP). OsSAP was both highly and rapidly expressed in response to drought stress. Additionally, OsSAP was found to be localized to the mitochondria. Overall, OsSAP represents a new type of Bax suppressor related gene and endows multiple stress tolerance in yeast.

Original language	English
Pages (from-to)	6113-6121
Number of pages	9
Journal	Molecular Biology Reports
Volume	40
Issue number	11
DOIs	https://doi.org/10.1007/s11033-013-2723-z
State	Published - Nov 2013

Keywords

Anti-apoptosis
Bax inhibitor
Rice
Yeast functional screen

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10.1007/s11033-013-2723-z

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title = "Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast",

abstract = "We identified rice genes that might be involved in drought stress tolerance by virtue of their anti-apoptotic activity. Potential anti-apoptosis related genes were identified by screening an Oryza sativa cDNA library derived from drought stressed tissues in a yeast functional assay. About 28 O. sativa cDNAs promoted yeast survival following engagement of Bax-induced apoptosis. An O. sativa cDNA encoding R12H780 was a highly conserved putative senescence-associated-protein (OsSAP). OsSAP was both highly and rapidly expressed in response to drought stress. Additionally, OsSAP was found to be localized to the mitochondria. Overall, OsSAP represents a new type of Bax suppressor related gene and endows multiple stress tolerance in yeast.",

keywords = "Anti-apoptosis, Bax inhibitor, Rice, Yeast functional screen",

author = "Mohammad Ubaidillah and Kim, \{Kyung A.\} and Kim, \{Yoon Ha\} and Lee, \{In Jung\} and Yun, \{Byung Wook\} and Kim, \{Doh Hoon\} and Loake, \{Gary J.\} and Kim, \{Kyung Min\}",

year = "2013",

month = nov,

doi = "10.1007/s11033-013-2723-z",

language = "English",

volume = "40",

pages = "6113--6121",

journal = "Molecular Biology Reports",

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TY - JOUR

T1 - Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast

AU - Ubaidillah, Mohammad

AU - Kim, Kyung A.

AU - Kim, Yoon Ha

AU - Lee, In Jung

AU - Yun, Byung Wook

AU - Kim, Doh Hoon

AU - Loake, Gary J.

AU - Kim, Kyung Min

PY - 2013/11

Y1 - 2013/11

N2 - We identified rice genes that might be involved in drought stress tolerance by virtue of their anti-apoptotic activity. Potential anti-apoptosis related genes were identified by screening an Oryza sativa cDNA library derived from drought stressed tissues in a yeast functional assay. About 28 O. sativa cDNAs promoted yeast survival following engagement of Bax-induced apoptosis. An O. sativa cDNA encoding R12H780 was a highly conserved putative senescence-associated-protein (OsSAP). OsSAP was both highly and rapidly expressed in response to drought stress. Additionally, OsSAP was found to be localized to the mitochondria. Overall, OsSAP represents a new type of Bax suppressor related gene and endows multiple stress tolerance in yeast.

AB - We identified rice genes that might be involved in drought stress tolerance by virtue of their anti-apoptotic activity. Potential anti-apoptosis related genes were identified by screening an Oryza sativa cDNA library derived from drought stressed tissues in a yeast functional assay. About 28 O. sativa cDNAs promoted yeast survival following engagement of Bax-induced apoptosis. An O. sativa cDNA encoding R12H780 was a highly conserved putative senescence-associated-protein (OsSAP). OsSAP was both highly and rapidly expressed in response to drought stress. Additionally, OsSAP was found to be localized to the mitochondria. Overall, OsSAP represents a new type of Bax suppressor related gene and endows multiple stress tolerance in yeast.

KW - Anti-apoptosis

KW - Bax inhibitor

KW - Rice

KW - Yeast functional screen

UR - https://www.scopus.com/pages/publications/84892764780

U2 - 10.1007/s11033-013-2723-z

DO - 10.1007/s11033-013-2723-z

M3 - Article

C2 - 24096889

AN - SCOPUS:84892764780

SN - 0301-4851

VL - 40

SP - 6113

EP - 6121

JO - Molecular Biology Reports

JF - Molecular Biology Reports

IS - 11

ER -

Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast

Abstract

Keywords

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