Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray

S. L. Yu; H. J. Chung; B. C. Sang; C. S. Park; J. H. Lee; D. H. Yoon; S. H. Lee; K. D. Choi

doi:10.1080/10495390701413391

Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray

S. L. Yu, H. J. Chung, B. C. Sang, C. S. Park, J. H. Lee, D. H. Yoon, S. H. Lee, K. D. Choi

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9 Scopus citations

Abstract

The 788-gene microarray was manufactured using selected elements from three different cDNA libraries in order to identify molecular processes that determine phenotypic characteristics between loin (M. longissimus thoracis) and round (M. semimembranosus) muscles. Microarray analyses identified 24 differentially expressed genes between the two muscles investigated. Five of the genes were verified by quantitative RT-PCR and three of them were mapped on bovine chromosomes using 5,000rad bovine radiation hybrid (RH) panel. The map locations indicated that they were mapped in the same chromosomal regions where IMF and growth QTLs were located, suggesting that they are most possible positional candidate genes for the traits.

Original language	English
Pages (from-to)	275-285
Number of pages	11
Journal	Animal Biotechnology
Volume	18
Issue number	4
DOIs	https://doi.org/10.1080/10495390701413391
State	Published - Oct 2007

Keywords

Cattle
cDNA microarray
Skeletal muscles

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10.1080/10495390701413391

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title = "Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray",

abstract = "The 788-gene microarray was manufactured using selected elements from three different cDNA libraries in order to identify molecular processes that determine phenotypic characteristics between loin (M. longissimus thoracis) and round (M. semimembranosus) muscles. Microarray analyses identified 24 differentially expressed genes between the two muscles investigated. Five of the genes were verified by quantitative RT-PCR and three of them were mapped on bovine chromosomes using 5,000rad bovine radiation hybrid (RH) panel. The map locations indicated that they were mapped in the same chromosomal regions where IMF and growth QTLs were located, suggesting that they are most possible positional candidate genes for the traits.",

keywords = "Cattle, cDNA microarray, Skeletal muscles",

author = "Yu, {S. L.} and Chung, {H. J.} and Sang, {B. C.} and Park, {C. S.} and Lee, {J. H.} and Yoon, {D. H.} and Lee, {S. H.} and Choi, {K. D.}",

year = "2007",

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T1 - Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray

AU - Yu, S. L.

AU - Chung, H. J.

AU - Sang, B. C.

AU - Park, C. S.

AU - Lee, J. H.

AU - Yoon, D. H.

AU - Lee, S. H.

AU - Choi, K. D.

PY - 2007/10

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AB - The 788-gene microarray was manufactured using selected elements from three different cDNA libraries in order to identify molecular processes that determine phenotypic characteristics between loin (M. longissimus thoracis) and round (M. semimembranosus) muscles. Microarray analyses identified 24 differentially expressed genes between the two muscles investigated. Five of the genes were verified by quantitative RT-PCR and three of them were mapped on bovine chromosomes using 5,000rad bovine radiation hybrid (RH) panel. The map locations indicated that they were mapped in the same chromosomal regions where IMF and growth QTLs were located, suggesting that they are most possible positional candidate genes for the traits.

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Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray

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