Identification of One of the Major Viruses Infecting Garlic Plants, Garlic Virus X

Sang Ik Song; Jong Tae Song; Moo Ung Chang; Jong Seob Lee; Yang Do Choi

Identification of One of the Major Viruses Infecting Garlic Plants, Garlic Virus X

Sang Ik Song, Jong Tae Song, Moo Ung Chang, Jong Seob Lee, Yang Do Choi

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21 Scopus citations

Abstract

A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic or streak symptoms. GVX belongs to an unassigned group of ShVX and GarV-type viruses rather than to carlaviruses or potexviruses. The recombinant CP of GVX was purified by Ni²⁺ -NTA affinity chromatography. Anti-GVX CP antibody was raised against the purified recombinant CP. GVX particle is flexuous, rod-shaped, and about 750 nm long as determined by immunoelectron microscopy. The extent of infection by GVX of garlic plants was analyzed by Northern or immunoblot analyses of individual garlic plants cultivated in different regions. These results showed that almost all of the garlic plants tested from 40 different regions including America, China, Japan, and Korea are infected with GVX.

Original language	English
Pages (from-to)	705-709
Number of pages	5
Journal	Molecules and Cells
Volume	7
Issue number	6
State	Published - 31 Dec 1997

Cite this

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title = "Identification of One of the Major Viruses Infecting Garlic Plants, Garlic Virus X",

abstract = "A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic or streak symptoms. GVX belongs to an unassigned group of ShVX and GarV-type viruses rather than to carlaviruses or potexviruses. The recombinant CP of GVX was purified by Ni2+ -NTA affinity chromatography. Anti-GVX CP antibody was raised against the purified recombinant CP. GVX particle is flexuous, rod-shaped, and about 750 nm long as determined by immunoelectron microscopy. The extent of infection by GVX of garlic plants was analyzed by Northern or immunoblot analyses of individual garlic plants cultivated in different regions. These results showed that almost all of the garlic plants tested from 40 different regions including America, China, Japan, and Korea are infected with GVX.",

author = "Song, {Sang Ik} and Song, {Jong Tae} and Chang, {Moo Ung} and Lee, {Jong Seob} and Choi, {Yang Do}",

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T1 - Identification of One of the Major Viruses Infecting Garlic Plants, Garlic Virus X

AU - Song, Sang Ik

AU - Song, Jong Tae

AU - Chang, Moo Ung

AU - Lee, Jong Seob

AU - Choi, Yang Do

PY - 1997/12/31

Y1 - 1997/12/31

N2 - A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic or streak symptoms. GVX belongs to an unassigned group of ShVX and GarV-type viruses rather than to carlaviruses or potexviruses. The recombinant CP of GVX was purified by Ni2+ -NTA affinity chromatography. Anti-GVX CP antibody was raised against the purified recombinant CP. GVX particle is flexuous, rod-shaped, and about 750 nm long as determined by immunoelectron microscopy. The extent of infection by GVX of garlic plants was analyzed by Northern or immunoblot analyses of individual garlic plants cultivated in different regions. These results showed that almost all of the garlic plants tested from 40 different regions including America, China, Japan, and Korea are infected with GVX.

AB - A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic or streak symptoms. GVX belongs to an unassigned group of ShVX and GarV-type viruses rather than to carlaviruses or potexviruses. The recombinant CP of GVX was purified by Ni2+ -NTA affinity chromatography. Anti-GVX CP antibody was raised against the purified recombinant CP. GVX particle is flexuous, rod-shaped, and about 750 nm long as determined by immunoelectron microscopy. The extent of infection by GVX of garlic plants was analyzed by Northern or immunoblot analyses of individual garlic plants cultivated in different regions. These results showed that almost all of the garlic plants tested from 40 different regions including America, China, Japan, and Korea are infected with GVX.

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JF - Molecules and Cells

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Identification of One of the Major Viruses Infecting Garlic Plants, Garlic Virus X

Abstract

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