Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

Won Hae Lee; Ki Woong Cho; Soo Young Park; Kee Sun Shin; Dong Sun Lee; Seon Kap Hwang; Seok Jong Seo; Jong Myeong Kim; Sa Youl Ghim; Bang Ho Song; Sang Han Lee; Jong Guk Kim

doi:10.4014/jmb.0800.102

Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

Won Hae Lee, Ki Woong Cho, Soo Young Park, Kee Sun Shin, Dong Sun Lee, Seon Kap Hwang, Seok Jong Seo, Jong Myeong Kim, Sa Youl Ghim, Bang Ho Song, Sang Han Lee, Jong Guk Kim

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207^T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

Original language	English
Pages (from-to)	1869-1873
Number of pages	5
Journal	Journal of Microbiology and Biotechnology
Volume	18
Issue number	12
DOIs	https://doi.org/10.4014/jmb.0800.102
State	Published - 28 Dec 2008

Keywords

16S rDNA
Eicosapentaenoic acid
Phylogenetic tree
Shewanella sp. KMG427

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10.4014/jmb.0800.102

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title = "Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer",

abstract = "An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.",

keywords = "16S rDNA, Eicosapentaenoic acid, Phylogenetic tree, Shewanella sp. KMG427",

author = "Lee, {Won Hae} and Cho, {Ki Woong} and Park, {Soo Young} and Shin, {Kee Sun} and Lee, {Dong Sun} and Hwang, {Seon Kap} and Seo, {Seok Jong} and Kim, {Jong Myeong} and Ghim, {Sa Youl} and Song, {Bang Ho} and Lee, {Sang Han} and Kim, {Jong Guk}",

year = "2008",

month = dec,

day = "28",

doi = "10.4014/jmb.0800.102",

language = "English",

volume = "18",

pages = "1869--1873",

journal = "Journal of Microbiology and Biotechnology",

issn = "1017-7825",

publisher = "Korean Society for Microbiolog and Biotechnology",

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T1 - Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

AU - Lee, Won Hae

AU - Cho, Ki Woong

AU - Park, Soo Young

AU - Shin, Kee Sun

AU - Lee, Dong Sun

AU - Hwang, Seon Kap

AU - Seo, Seok Jong

AU - Kim, Jong Myeong

AU - Ghim, Sa Youl

AU - Song, Bang Ho

AU - Lee, Sang Han

AU - Kim, Jong Guk

PY - 2008/12/28

Y1 - 2008/12/28

N2 - An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

AB - An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

KW - 16S rDNA

KW - Eicosapentaenoic acid

KW - Phylogenetic tree

KW - Shewanella sp. KMG427

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DO - 10.4014/jmb.0800.102

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AN - SCOPUS:67651068117

SN - 1017-7825

VL - 18

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EP - 1873

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

IS - 12

ER -

Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

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Keywords

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