Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

Won Hae Lee; Ki Woong Cho; Soo Young Park; Kee Sun Shin; Dong Sun Lee; Seon Kap Hwang; Seok Jong Seo; Jong Myeong Kim; Sa Youl Ghim; Bang Ho Song; Sang Han Lee; Jong Guk Kim

doi:10.4014/jmb.0800.102

Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

Won Hae Lee
, Ki Woong Cho
, Soo Young Park
, Kee Sun Shin
, Dong Sun Lee
, Seon Kap Hwang
, Seok Jong Seo
, Jong Myeong Kim
, Sa Youl Ghim
, Bang Ho Song
, Sang Han Lee
, Jong Guk Kim

Kyungpook National University
Anyang University
Korea Research Institute of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207^T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

Original language	English
Pages (from-to)	1869-1873
Number of pages	5
Journal	Journal of Microbiology and Biotechnology
Volume	18
Issue number	12
DOIs	https://doi.org/10.4014/jmb.0800.102
State	Published - 28 Dec 2008

Keywords

16S rDNA
Eicosapentaenoic acid
Phylogenetic tree
Shewanella sp. KMG427

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10.4014/jmb.0800.102

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@article{1ca17ee1850041c5a4b21394d523fbf2,

title = "Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer",

abstract = "An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10\% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100\% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.",

keywords = "16S rDNA, Eicosapentaenoic acid, Phylogenetic tree, Shewanella sp. KMG427",

author = "Lee, \{Won Hae\} and Cho, \{Ki Woong\} and Park, \{Soo Young\} and Shin, \{Kee Sun\} and Lee, \{Dong Sun\} and Hwang, \{Seon Kap\} and Seo, \{Seok Jong\} and Kim, \{Jong Myeong\} and Ghim, \{Sa Youl\} and Song, \{Bang Ho\} and Lee, \{Sang Han\} and Kim, \{Jong Guk\}",

year = "2008",

month = dec,

day = "28",

doi = "10.4014/jmb.0800.102",

language = "English",

volume = "18",

pages = "1869--1873",

journal = "Journal of Microbiology and Biotechnology",

issn = "1017-7825",

publisher = "Korean Society for Microbiolog and Biotechnology",

number = "12",

}

TY - JOUR

T1 - Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

AU - Lee, Won Hae

AU - Cho, Ki Woong

AU - Park, Soo Young

AU - Shin, Kee Sun

AU - Lee, Dong Sun

AU - Hwang, Seon Kap

AU - Seo, Seok Jong

AU - Kim, Jong Myeong

AU - Ghim, Sa Youl

AU - Song, Bang Ho

AU - Lee, Sang Han

AU - Kim, Jong Guk

PY - 2008/12/28

Y1 - 2008/12/28

N2 - An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

AB - An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4°C after cell proliferation at 20°C. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

KW - 16S rDNA

KW - Eicosapentaenoic acid

KW - Phylogenetic tree

KW - Shewanella sp. KMG427

UR - https://www.scopus.com/pages/publications/67651068117

U2 - 10.4014/jmb.0800.102

DO - 10.4014/jmb.0800.102

M3 - Article

C2 - 19131686

AN - SCOPUS:67651068117

SN - 1017-7825

VL - 18

SP - 1869

EP - 1873

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

IS - 12

ER -

Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

Abstract

Keywords

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