TY - JOUR
T1 - Identification of Taenia asiatica in China
T2 - Molecular, morphological, and epidemiological analysis of a Luzhai isolate
AU - Eom, Keeseon S.
AU - Jeon, Hyung Kyu
AU - Kong, Yoon
AU - Hwang, Ui Wook
AU - Yang, Yichao
AU - Li, Xueming
AU - Xu, Longqi
AU - Feng, Zheng
AU - Pawlowski, Zbigniew S.
AU - Rim, Han Jong
PY - 2002
Y1 - 2002
N2 - Multiple analysis has characterized a recently described tapeworm of people, Taenia asiatica, in mainland China. Six adult tapeworms collected from people of the Zhuang minority residing in the southern part of China (Luzhai isolate) were comparatively analyzed with other tapeworms from people: T. asiatica (n = 2, South Korea), T. saginata (n = 1, Poland; n = 1, Korea), and T. solium (n = 1, People's Republic of China). Experimental infections with eggs from the Luzhai isolate in pigs and cattle produced cysticerci, each with a hookletless scolex and with wartlike formations on the external surface of the bladder wall. There were rostellar protrusions in the scolices of adult worms. Random amplified polymorphic DNA analysis using 3 arbitrary primers produced bands identical to those of the Korean T. asiatica. Conversely, T. saginata and T. solium exhibited different banding patterns. Phylogenetic relationships inferred from the complete nucleotide sequences of the internal transcribed spacer 2 placed the Chinese tapeworms consistently within the T. asiatica clade by 96% bootstrapping value in the maximum likelihood analysis, 96% in maximum parsimony, and 100% in neighbor joining. These collective data demonstrate that T. asiatica is sympatrically distributed with the other 2 species of Taenia in the human host in mainland China.
AB - Multiple analysis has characterized a recently described tapeworm of people, Taenia asiatica, in mainland China. Six adult tapeworms collected from people of the Zhuang minority residing in the southern part of China (Luzhai isolate) were comparatively analyzed with other tapeworms from people: T. asiatica (n = 2, South Korea), T. saginata (n = 1, Poland; n = 1, Korea), and T. solium (n = 1, People's Republic of China). Experimental infections with eggs from the Luzhai isolate in pigs and cattle produced cysticerci, each with a hookletless scolex and with wartlike formations on the external surface of the bladder wall. There were rostellar protrusions in the scolices of adult worms. Random amplified polymorphic DNA analysis using 3 arbitrary primers produced bands identical to those of the Korean T. asiatica. Conversely, T. saginata and T. solium exhibited different banding patterns. Phylogenetic relationships inferred from the complete nucleotide sequences of the internal transcribed spacer 2 placed the Chinese tapeworms consistently within the T. asiatica clade by 96% bootstrapping value in the maximum likelihood analysis, 96% in maximum parsimony, and 100% in neighbor joining. These collective data demonstrate that T. asiatica is sympatrically distributed with the other 2 species of Taenia in the human host in mainland China.
UR - http://www.scopus.com/inward/record.url?scp=0036344643&partnerID=8YFLogxK
U2 - 10.1645/0022-3395(2002)088[0758:IOTAIC]2.0.CO;2
DO - 10.1645/0022-3395(2002)088[0758:IOTAIC]2.0.CO;2
M3 - Article
AN - SCOPUS:0036344643
SN - 0022-3395
VL - 88
SP - 758
EP - 764
JO - Journal of Parasitology
JF - Journal of Parasitology
IS - 4
ER -