IFT46 plays crucial roles in craniofacial and cilia development

Inji Park; Hyun Kyung Lee; Chowon Kim; Tayaba Ismail; Yoo Kyung Kim; Jeen Woo Park; Oh Shin Kwon; Beom Sik Kang; Dong Seok Lee; Tae Joo Park; Mae Ja Park; Sun Cheol Choi; Hyun Shik Lee

doi:10.1016/j.bbrc.2016.06.083

IFT46 plays crucial roles in craniofacial and cilia development

Inji Park, Hyun Kyung Lee, Chowon Kim, Tayaba Ismail, Yoo Kyung Kim, Jeen Woo Park, Oh Shin Kwon, Beom Sik Kang, Dong Seok Lee, Tae Joo Park, Mae Ja Park, Sun Cheol Choi, Hyun Shik Lee

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The intraflagellar transport (IFT) system is essential for bidirectional movement of ciliary components from the basal body to the tip beneath the ciliary sheath and is conserved for cilia and flagella formation in most vertebrates. IFT complex A is involved in anterograde trafficking, whereas complex B is involved in retrograde trafficking. IFT46 is well known as a crucial component of IFT complex B, however, its developmental functions are poorly understood. In this study, we investigated the novel functions of IFT46 during vertebrate development, especially, ciliogenesis and neurogenesis, because IFT46 is strongly expressed in both multiciliated cells of epithelial and neural tissues. Knockdown of IFT46 using morpholino microinjections caused shortening of the body axis as well as the formation of fewer and shorter cilia. Furthermore, loss of IFT46 down-regulated the expression of the neural plate and neural tube markers, thus may influence Wnt/planar cell polarity and the sonic hedgehog signaling pathway during neurogenesis. In addition, loss of IFT46 caused craniofacial defects by interfering with cartilage formation. In conclusion, our results depict that IFT46 plays important roles in cilia as well as in neural and craniofacial development.

Original language	English
Pages (from-to)	419-425
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	477
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2016.06.083
State	Published - 26 Aug 2016

Keywords

Ciliogenesis
Craniofacial development
IFT46
Neurogenesis

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10.1016/j.bbrc.2016.06.083

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title = "IFT46 plays crucial roles in craniofacial and cilia development",

abstract = "The intraflagellar transport (IFT) system is essential for bidirectional movement of ciliary components from the basal body to the tip beneath the ciliary sheath and is conserved for cilia and flagella formation in most vertebrates. IFT complex A is involved in anterograde trafficking, whereas complex B is involved in retrograde trafficking. IFT46 is well known as a crucial component of IFT complex B, however, its developmental functions are poorly understood. In this study, we investigated the novel functions of IFT46 during vertebrate development, especially, ciliogenesis and neurogenesis, because IFT46 is strongly expressed in both multiciliated cells of epithelial and neural tissues. Knockdown of IFT46 using morpholino microinjections caused shortening of the body axis as well as the formation of fewer and shorter cilia. Furthermore, loss of IFT46 down-regulated the expression of the neural plate and neural tube markers, thus may influence Wnt/planar cell polarity and the sonic hedgehog signaling pathway during neurogenesis. In addition, loss of IFT46 caused craniofacial defects by interfering with cartilage formation. In conclusion, our results depict that IFT46 plays important roles in cilia as well as in neural and craniofacial development.",

keywords = "Ciliogenesis, Craniofacial development, IFT46, Neurogenesis",

author = "Inji Park and Lee, {Hyun Kyung} and Chowon Kim and Tayaba Ismail and Kim, {Yoo Kyung} and Park, {Jeen Woo} and Kwon, {Oh Shin} and Kang, {Beom Sik} and Lee, {Dong Seok} and Park, {Tae Joo} and Park, {Mae Ja} and Choi, {Sun Cheol} and Lee, {Hyun Shik}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2016",

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doi = "10.1016/j.bbrc.2016.06.083",

language = "English",

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T1 - IFT46 plays crucial roles in craniofacial and cilia development

AU - Park, Inji

AU - Lee, Hyun Kyung

AU - Kim, Chowon

AU - Ismail, Tayaba

AU - Kim, Yoo Kyung

AU - Park, Jeen Woo

AU - Kwon, Oh Shin

AU - Kang, Beom Sik

AU - Lee, Dong Seok

AU - Park, Tae Joo

AU - Park, Mae Ja

AU - Choi, Sun Cheol

AU - Lee, Hyun Shik

PY - 2016/8/26

Y1 - 2016/8/26

N2 - The intraflagellar transport (IFT) system is essential for bidirectional movement of ciliary components from the basal body to the tip beneath the ciliary sheath and is conserved for cilia and flagella formation in most vertebrates. IFT complex A is involved in anterograde trafficking, whereas complex B is involved in retrograde trafficking. IFT46 is well known as a crucial component of IFT complex B, however, its developmental functions are poorly understood. In this study, we investigated the novel functions of IFT46 during vertebrate development, especially, ciliogenesis and neurogenesis, because IFT46 is strongly expressed in both multiciliated cells of epithelial and neural tissues. Knockdown of IFT46 using morpholino microinjections caused shortening of the body axis as well as the formation of fewer and shorter cilia. Furthermore, loss of IFT46 down-regulated the expression of the neural plate and neural tube markers, thus may influence Wnt/planar cell polarity and the sonic hedgehog signaling pathway during neurogenesis. In addition, loss of IFT46 caused craniofacial defects by interfering with cartilage formation. In conclusion, our results depict that IFT46 plays important roles in cilia as well as in neural and craniofacial development.

AB - The intraflagellar transport (IFT) system is essential for bidirectional movement of ciliary components from the basal body to the tip beneath the ciliary sheath and is conserved for cilia and flagella formation in most vertebrates. IFT complex A is involved in anterograde trafficking, whereas complex B is involved in retrograde trafficking. IFT46 is well known as a crucial component of IFT complex B, however, its developmental functions are poorly understood. In this study, we investigated the novel functions of IFT46 during vertebrate development, especially, ciliogenesis and neurogenesis, because IFT46 is strongly expressed in both multiciliated cells of epithelial and neural tissues. Knockdown of IFT46 using morpholino microinjections caused shortening of the body axis as well as the formation of fewer and shorter cilia. Furthermore, loss of IFT46 down-regulated the expression of the neural plate and neural tube markers, thus may influence Wnt/planar cell polarity and the sonic hedgehog signaling pathway during neurogenesis. In addition, loss of IFT46 caused craniofacial defects by interfering with cartilage formation. In conclusion, our results depict that IFT46 plays important roles in cilia as well as in neural and craniofacial development.

KW - Ciliogenesis

KW - Craniofacial development

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KW - Neurogenesis

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JO - Biochemical and Biophysical Research Communications

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IFT46 plays crucial roles in craniofacial and cilia development

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