Imipenem-resistant Achromobacter xylosoxidans carrying bla VIM-2-containing class 1 integron

Kyeong Seob Shin; Kyudong Han; Jungnam Lee; Seung Bok Hong; Bo Ra Son; Sei Jin Youn; Jungmin Kim; Hyung Sik Shin

doi:10.1016/j.diagmicrobio.2005.06.018

Imipenem-resistant Achromobacter xylosoxidans carrying bla _VIM-2-containing class 1 integron

Kyeong Seob Shin, Kyudong Han, Jungnam Lee, Seung Bok Hong, Bo Ra Son, Sei Jin Youn, Jungmin Kim, Hyung Sik Shin

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We characterized seven isolates of imipenem-resistant Achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in Korea during 2001 to 2003. From the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce VIM-2, OXA-30, and chromosomal AmpC β-lactamase with a pI of 8.4. They showed a similar antibiogram, which were resistant to all tested aminoglycosides as well as β-lactams including imipenem (16-32 mg/L) and aztreonam (128 mg/L), and a same DNA fingerprinting pattern by random amplified polymorphic DNA analysis, suggesting that these originated from a single clone. From the analysis of integron structure carried by an isolate of A. xylosoxidans CBU1760, bla_VIM-2 was found to be part of a gene cassette carried on a class 1 integron (3.4 kb) containing three aacA4 gene cassettes. This is the first report of bla_VIM-2 in A. xylosoxidans.

Original language	English
Pages (from-to)	215-220
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	53
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2005.06.018
State	Published - Nov 2005

Keywords

Achromobacter xylosoxidans
Integron
Metallo-β-lactamase
VIM-2

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2005.06.018

Cite this

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title = "Imipenem-resistant Achromobacter xylosoxidans carrying bla VIM-2-containing class 1 integron",

abstract = "We characterized seven isolates of imipenem-resistant Achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in Korea during 2001 to 2003. From the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce VIM-2, OXA-30, and chromosomal AmpC β-lactamase with a pI of 8.4. They showed a similar antibiogram, which were resistant to all tested aminoglycosides as well as β-lactams including imipenem (16-32 mg/L) and aztreonam (128 mg/L), and a same DNA fingerprinting pattern by random amplified polymorphic DNA analysis, suggesting that these originated from a single clone. From the analysis of integron structure carried by an isolate of A. xylosoxidans CBU1760, blaVIM-2 was found to be part of a gene cassette carried on a class 1 integron (3.4 kb) containing three aacA4 gene cassettes. This is the first report of blaVIM-2 in A. xylosoxidans.",

keywords = "Achromobacter xylosoxidans, Integron, Metallo-β-lactamase, VIM-2",

author = "Shin, {Kyeong Seob} and Kyudong Han and Jungnam Lee and Hong, {Seung Bok} and Son, {Bo Ra} and Youn, {Sei Jin} and Jungmin Kim and Shin, {Hyung Sik}",

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AU - Shin, Kyeong Seob

AU - Han, Kyudong

AU - Lee, Jungnam

AU - Hong, Seung Bok

AU - Son, Bo Ra

AU - Youn, Sei Jin

AU - Kim, Jungmin

AU - Shin, Hyung Sik

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N2 - We characterized seven isolates of imipenem-resistant Achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in Korea during 2001 to 2003. From the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce VIM-2, OXA-30, and chromosomal AmpC β-lactamase with a pI of 8.4. They showed a similar antibiogram, which were resistant to all tested aminoglycosides as well as β-lactams including imipenem (16-32 mg/L) and aztreonam (128 mg/L), and a same DNA fingerprinting pattern by random amplified polymorphic DNA analysis, suggesting that these originated from a single clone. From the analysis of integron structure carried by an isolate of A. xylosoxidans CBU1760, blaVIM-2 was found to be part of a gene cassette carried on a class 1 integron (3.4 kb) containing three aacA4 gene cassettes. This is the first report of blaVIM-2 in A. xylosoxidans.

AB - We characterized seven isolates of imipenem-resistant Achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in Korea during 2001 to 2003. From the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce VIM-2, OXA-30, and chromosomal AmpC β-lactamase with a pI of 8.4. They showed a similar antibiogram, which were resistant to all tested aminoglycosides as well as β-lactams including imipenem (16-32 mg/L) and aztreonam (128 mg/L), and a same DNA fingerprinting pattern by random amplified polymorphic DNA analysis, suggesting that these originated from a single clone. From the analysis of integron structure carried by an isolate of A. xylosoxidans CBU1760, blaVIM-2 was found to be part of a gene cassette carried on a class 1 integron (3.4 kb) containing three aacA4 gene cassettes. This is the first report of blaVIM-2 in A. xylosoxidans.

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