Immobilization of a cyclodextrin glucanotransferase (CGTase) onto polyethylene film with a carboxylic acid group and production of cyclodextrins from corn starch using CGTase-immobilized PE film

Seong Ho Choi, Min Seok Kim, Jae Jeong Ryoo, Kwang Pill Lee, Hyun Dong Shin, Sun Hwa Kim, Yong Hyun Lee

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Carboxylic acid groups were introduced onto polyethylene (PE) film by radiation-induced graft copolymerization. Subsequently, the clodextrin glucanotransferase (CGTase) was immobilized on the PE film with a carboxylic acid group. The activity of the immobilized CGTase on PE film was in the range of 0.40-1.04 U/cm2 per min. The production of cyclodextrins (CDs) from corn starch was examined using the CGTase-immobilized PE film. The production ratios of CDs using CGTase-immobilized PE film was in the following order: α-CD > β-CD > γ-CD.

Original languageEnglish
Pages (from-to)2451-2457
Number of pages7
JournalJournal of Applied Polymer Science
Volume85
Issue number11
DOIs
StatePublished - 12 Sep 2002

Keywords

  • Acrylic acid
  • Cyclodextrin glucanotransferase (CGTase)
  • Cyclodextrins (CDs)
  • Immobilization
  • Polyethylene film
  • Radiation-induced grafting copolymerization

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