Immobilization of antibody on a cyclic olefin copolymer surface with functionalizable, non-biofouling poly[oligo(ethylene glycol) methacrylate]

Seung Pyo Jeong; Sung Min Kang; Daewha Hong; Hee Yoon Lee; Insung S. Choi; Sangwon Ko; Jungkyu K. Lee

doi:10.1166/jnn.2015.9335

Immobilization of antibody on a cyclic olefin copolymer surface with functionalizable, non-biofouling poly[oligo(ethylene glycol) methacrylate]

Seung Pyo Jeong, Sung Min Kang, Daewha Hong, Hee Yoon Lee, Insung S. Choi, Sangwon Ko, Jungkyu K. Lee

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We report a perfluoroaryl azide-based photoreaction for synthesizing functionalizable and non-biofouling poly[oligo(ethylene glycol) methacrylate] (pOEGMA) films on a chemically inert COC substrate, and an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles. The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.

Original language	English
Article number	A132
Pages (from-to)	1767-1770
Number of pages	4
Journal	Journal of Nanoscience and Nanotechnology
Volume	15
Issue number	2
DOIs	https://doi.org/10.1166/jnn.2015.9335
State	Published - 1 Mar 2015

Keywords

Non-biofouling polymer
Perfluoroaryl azide-based photopolymerization

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10.1166/jnn.2015.9335

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title = "Immobilization of antibody on a cyclic olefin copolymer surface with functionalizable, non-biofouling poly[oligo(ethylene glycol) methacrylate]",

abstract = "We report a perfluoroaryl azide-based photoreaction for synthesizing functionalizable and non-biofouling poly[oligo(ethylene glycol) methacrylate] (pOEGMA) films on a chemically inert COC substrate, and an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles. The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.",

keywords = "Non-biofouling polymer, Perfluoroaryl azide-based photopolymerization",

author = "Jeong, {Seung Pyo} and Kang, {Sung Min} and Daewha Hong and Lee, {Hee Yoon} and Choi, {Insung S.} and Sangwon Ko and Lee, {Jungkyu K.}",

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AU - Jeong, Seung Pyo

AU - Kang, Sung Min

AU - Hong, Daewha

AU - Lee, Hee Yoon

AU - Choi, Insung S.

AU - Ko, Sangwon

AU - Lee, Jungkyu K.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - We report a perfluoroaryl azide-based photoreaction for synthesizing functionalizable and non-biofouling poly[oligo(ethylene glycol) methacrylate] (pOEGMA) films on a chemically inert COC substrate, and an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles. The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.

AB - We report a perfluoroaryl azide-based photoreaction for synthesizing functionalizable and non-biofouling poly[oligo(ethylene glycol) methacrylate] (pOEGMA) films on a chemically inert COC substrate, and an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles. The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.

KW - Non-biofouling polymer

KW - Perfluoroaryl azide-based photopolymerization

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DO - 10.1166/jnn.2015.9335

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JO - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

IS - 2

M1 - A132

ER -

Immobilization of antibody on a cyclic olefin copolymer surface with functionalizable, non-biofouling poly[oligo(ethylene glycol) methacrylate]

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