Immobilization of antibody on a cyclic olefin copolymer surface with functionalizable, non-biofouling poly[oligo(ethylene glycol) methacrylate]

We report a perfluoroaryl azide-based photoreaction for synthesizing functionalizable and non-biofouling poly[oligo(ethylene glycol) methacrylate] (pOEGMA) films on a chemically inert COC substrate, and an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles. The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.