Immune-enhancing effects of a high molecular weight fraction of Cynanchum wilfordii hemsley in macrophages and immunosuppressed mice

Mi Jang; Tae Gyu Lim; Sungeun Ahn; Hee Do Hong; Young Kyoung Rhee; Kyung Tack Kim; Eunjung Lee; Jeong Hoon Lee; Yun Ji Lee; Chan Sik Jung; Dae Young Lee; Chang Won Cho

doi:10.3390/nu8100600

Immune-enhancing effects of a high molecular weight fraction of Cynanchum wilfordii hemsley in macrophages and immunosuppressed mice

Mi Jang, Tae Gyu Lim, Sungeun Ahn, Hee Do Hong, Young Kyoung Rhee, Kyung Tack Kim, Eunjung Lee, Jeong Hoon Lee, Yun Ji Lee, Chan Sik Jung, Dae Young Lee, Chang Won Cho

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The objective of this study was to investigate the immune-enhancing activity of a high molecular weight fraction (HMF) of Cynanchum wilfordii in RAW 264.7 macrophages and the cyclophosphamide (CYC)-induced mouse model of immunosuppression. To identify the bioactive substances of HMF, a crude polysaccharide (HMFO) was obtained and treated with sodium periodate (an oxidation agent) or digested with protease. In macrophages, HMF treatment enhanced the production of nitric oxide (NO) and cytokines (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)), as well as phagocytic ability. In CYC-immunosuppressed mice, HMF improved relative spleen and thymus weights, natural killer (NK) cell activity, and splenic lymphocyte proliferation. These increases in NO and cytokines were mediated by up-regulation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Periodate treatment, but not protease treatment, decreased the immune-enhancing activity of HMFO, suggesting that polysaccharides are the active ingredients in C. wilfordii extract.

Original language	English
Article number	600
Journal	Nutrients
Volume	8
Issue number	10
DOIs	https://doi.org/10.3390/nu8100600
State	Published - Oct 2016

Keywords

Cyclophosphamide
Cynanchum wilfordii
Cytokine
Immunostimulatory activity
Nitric oxide
Polysaccharide

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title = "Immune-enhancing effects of a high molecular weight fraction of Cynanchum wilfordii hemsley in macrophages and immunosuppressed mice",

abstract = "The objective of this study was to investigate the immune-enhancing activity of a high molecular weight fraction (HMF) of Cynanchum wilfordii in RAW 264.7 macrophages and the cyclophosphamide (CYC)-induced mouse model of immunosuppression. To identify the bioactive substances of HMF, a crude polysaccharide (HMFO) was obtained and treated with sodium periodate (an oxidation agent) or digested with protease. In macrophages, HMF treatment enhanced the production of nitric oxide (NO) and cytokines (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)), as well as phagocytic ability. In CYC-immunosuppressed mice, HMF improved relative spleen and thymus weights, natural killer (NK) cell activity, and splenic lymphocyte proliferation. These increases in NO and cytokines were mediated by up-regulation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Periodate treatment, but not protease treatment, decreased the immune-enhancing activity of HMFO, suggesting that polysaccharides are the active ingredients in C. wilfordii extract.",

keywords = "Cyclophosphamide, Cynanchum wilfordii, Cytokine, Immunostimulatory activity, Nitric oxide, Polysaccharide",

author = "Mi Jang and Lim, {Tae Gyu} and Sungeun Ahn and Hong, {Hee Do} and Rhee, {Young Kyoung} and Kim, {Kyung Tack} and Eunjung Lee and Lee, {Jeong Hoon} and Lee, {Yun Ji} and Jung, {Chan Sik} and Lee, {Dae Young} and Cho, {Chang Won}",

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year = "2016",

month = oct,

doi = "10.3390/nu8100600",

language = "English",

volume = "8",

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AU - Jang, Mi

AU - Lim, Tae Gyu

AU - Ahn, Sungeun

AU - Hong, Hee Do

AU - Rhee, Young Kyoung

AU - Kim, Kyung Tack

AU - Lee, Eunjung

AU - Lee, Jeong Hoon

AU - Lee, Yun Ji

AU - Jung, Chan Sik

AU - Lee, Dae Young

AU - Cho, Chang Won

PY - 2016/10

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AB - The objective of this study was to investigate the immune-enhancing activity of a high molecular weight fraction (HMF) of Cynanchum wilfordii in RAW 264.7 macrophages and the cyclophosphamide (CYC)-induced mouse model of immunosuppression. To identify the bioactive substances of HMF, a crude polysaccharide (HMFO) was obtained and treated with sodium periodate (an oxidation agent) or digested with protease. In macrophages, HMF treatment enhanced the production of nitric oxide (NO) and cytokines (tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β)), as well as phagocytic ability. In CYC-immunosuppressed mice, HMF improved relative spleen and thymus weights, natural killer (NK) cell activity, and splenic lymphocyte proliferation. These increases in NO and cytokines were mediated by up-regulation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Periodate treatment, but not protease treatment, decreased the immune-enhancing activity of HMFO, suggesting that polysaccharides are the active ingredients in C. wilfordii extract.

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Immune-enhancing effects of a high molecular weight fraction of Cynanchum wilfordii hemsley in macrophages and immunosuppressed mice

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Keywords

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