Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus

Hye Young Jeoung; Won Ha Lee; Wooseog Jeong; Young Joon Ko; Cheong Up Choi; Dong Jun An

doi:10.1016/j.rvsc.2010.03.012

Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus

Hye Young Jeoung, Won Ha Lee, Wooseog Jeong, Young Joon Ko, Cheong Up Choi, Dong Jun An

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Virus-like particles (VLPs) are particles that consist of viral capsid proteins and are structurally similar to authentic virus. To express VLPs of the porcine encephalomyocarditis virus (EMCV) and investigate their efficacy and immuno response in vivo, a plasmid (P12A3C-pCI) containing the P12A and 3C genes of the EMCV-K3 viral strain was constructed. The VLPs of EMCV-K3 were successfully assembled in 293FT cells on 3 days after transfection with P12A3C-pCI and were identified as particles of about 30-40. nm using transmission electron microscopy (TEM). In an in vivo experiment, the murine cytokines induced by VLPs of naked DNA vaccine showed that the Th1 indicators IL-2, TNF-α and GM-CSF, and the Th2 indicators IL-4 and IL-10 were increased. The immunization of mice with the P12A3C-pCI plasmid induced high levels of neutralizing antibody from 128- to 256-fold and led to a significant protection ratio (90%) after challenge with EMCV-K3 (wild-type strain). These VLPs may represent a novel vaccine strategy for the control of EMCV infection on pig farms.

Original language	English
Pages (from-to)	295-300
Number of pages	6
Journal	Research in Veterinary Science
Volume	89
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2010.03.012
State	Published - Oct 2010

Keywords

DNA vaccine
EMCV
Virus-like particle

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.rvsc.2010.03.012

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title = "Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus",

abstract = "Virus-like particles (VLPs) are particles that consist of viral capsid proteins and are structurally similar to authentic virus. To express VLPs of the porcine encephalomyocarditis virus (EMCV) and investigate their efficacy and immuno response in vivo, a plasmid (P12A3C-pCI) containing the P12A and 3C genes of the EMCV-K3 viral strain was constructed. The VLPs of EMCV-K3 were successfully assembled in 293FT cells on 3 days after transfection with P12A3C-pCI and were identified as particles of about 30-40. nm using transmission electron microscopy (TEM). In an in vivo experiment, the murine cytokines induced by VLPs of naked DNA vaccine showed that the Th1 indicators IL-2, TNF-α and GM-CSF, and the Th2 indicators IL-4 and IL-10 were increased. The immunization of mice with the P12A3C-pCI plasmid induced high levels of neutralizing antibody from 128- to 256-fold and led to a significant protection ratio (90%) after challenge with EMCV-K3 (wild-type strain). These VLPs may represent a novel vaccine strategy for the control of EMCV infection on pig farms.",

keywords = "DNA vaccine, EMCV, Virus-like particle",

author = "Jeoung, {Hye Young} and Lee, {Won Ha} and Wooseog Jeong and Ko, {Young Joon} and Choi, {Cheong Up} and An, {Dong Jun}",

year = "2010",

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language = "English",

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T1 - Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus

AU - Jeoung, Hye Young

AU - Lee, Won Ha

AU - Jeong, Wooseog

AU - Ko, Young Joon

AU - Choi, Cheong Up

AU - An, Dong Jun

PY - 2010/10

Y1 - 2010/10

N2 - Virus-like particles (VLPs) are particles that consist of viral capsid proteins and are structurally similar to authentic virus. To express VLPs of the porcine encephalomyocarditis virus (EMCV) and investigate their efficacy and immuno response in vivo, a plasmid (P12A3C-pCI) containing the P12A and 3C genes of the EMCV-K3 viral strain was constructed. The VLPs of EMCV-K3 were successfully assembled in 293FT cells on 3 days after transfection with P12A3C-pCI and were identified as particles of about 30-40. nm using transmission electron microscopy (TEM). In an in vivo experiment, the murine cytokines induced by VLPs of naked DNA vaccine showed that the Th1 indicators IL-2, TNF-α and GM-CSF, and the Th2 indicators IL-4 and IL-10 were increased. The immunization of mice with the P12A3C-pCI plasmid induced high levels of neutralizing antibody from 128- to 256-fold and led to a significant protection ratio (90%) after challenge with EMCV-K3 (wild-type strain). These VLPs may represent a novel vaccine strategy for the control of EMCV infection on pig farms.

AB - Virus-like particles (VLPs) are particles that consist of viral capsid proteins and are structurally similar to authentic virus. To express VLPs of the porcine encephalomyocarditis virus (EMCV) and investigate their efficacy and immuno response in vivo, a plasmid (P12A3C-pCI) containing the P12A and 3C genes of the EMCV-K3 viral strain was constructed. The VLPs of EMCV-K3 were successfully assembled in 293FT cells on 3 days after transfection with P12A3C-pCI and were identified as particles of about 30-40. nm using transmission electron microscopy (TEM). In an in vivo experiment, the murine cytokines induced by VLPs of naked DNA vaccine showed that the Th1 indicators IL-2, TNF-α and GM-CSF, and the Th2 indicators IL-4 and IL-10 were increased. The immunization of mice with the P12A3C-pCI plasmid induced high levels of neutralizing antibody from 128- to 256-fold and led to a significant protection ratio (90%) after challenge with EMCV-K3 (wild-type strain). These VLPs may represent a novel vaccine strategy for the control of EMCV infection on pig farms.

KW - DNA vaccine

KW - EMCV

KW - Virus-like particle

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JO - Research in Veterinary Science

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Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus

Abstract

Keywords

UN SDGs

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