Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas

Eun Ik Son; Il Man Kim; Dong Won Kim; Man Bin Yim; Yu Na Kang; Sang Sook Lee; Kun Young Kwon; Seong Il Suh; Taeg Kyu Kwon; Jung Jeung Lee; Dong Sug Kim; Sang Pyo Kim

doi:10.1046/j.1440-1827.2003.01444.x

Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas

Eun Ik Son, Il Man Kim, Dong Won Kim, Man Bin Yim, Yu Na Kang, Sang Sook Lee, Kun Young Kwon, Seong Il Suh, Taeg Kyu Kwon, Jung Jeung Lee, Dong Sug Kim, Sang Pyo Kim

Department of Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non-anaplastic, four diffuse/anaplastic, three nodular/non-anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl-2, and Ki-67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non-anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl-2. In addition, Ki-67 labeling indices for anaplastic subtypes (39.0 ± 7.42%) were significantly higher than that of non-anaplastic medulloblastomas (11.4 ± 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.

Original language	English
Pages (from-to)	67-73
Number of pages	7
Journal	Pathology International
Volume	53
Issue number	2
DOIs	https://doi.org/10.1046/j.1440-1827.2003.01444.x
State	Published - 1 Feb 2003

Keywords

Anaplasia
Histopathology
Immunohistochemistry
Medulloblastoma
Pediatric
Subtype

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10.1046/j.1440-1827.2003.01444.x

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title = "Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas",

abstract = "Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non-anaplastic, four diffuse/anaplastic, three nodular/non-anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl-2, and Ki-67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non-anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl-2. In addition, Ki-67 labeling indices for anaplastic subtypes (39.0 ± 7.42%) were significantly higher than that of non-anaplastic medulloblastomas (11.4 ± 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.",

keywords = "Anaplasia, Histopathology, Immunohistochemistry, Medulloblastoma, Pediatric, Subtype",

author = "Son, {Eun Ik} and Kim, {Il Man} and Kim, {Dong Won} and Yim, {Man Bin} and Kang, {Yu Na} and Lee, {Sang Sook} and Kwon, {Kun Young} and Suh, {Seong Il} and Kwon, {Taeg Kyu} and Lee, {Jung Jeung} and Kim, {Dong Sug} and Kim, {Sang Pyo}",

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AU - Son, Eun Ik

AU - Kim, Il Man

AU - Kim, Dong Won

AU - Yim, Man Bin

AU - Kang, Yu Na

AU - Lee, Sang Sook

AU - Kwon, Kun Young

AU - Suh, Seong Il

AU - Kwon, Taeg Kyu

AU - Lee, Jung Jeung

AU - Kim, Dong Sug

AU - Kim, Sang Pyo

PY - 2003/2/1

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N2 - Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non-anaplastic, four diffuse/anaplastic, three nodular/non-anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl-2, and Ki-67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non-anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl-2. In addition, Ki-67 labeling indices for anaplastic subtypes (39.0 ± 7.42%) were significantly higher than that of non-anaplastic medulloblastomas (11.4 ± 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.

AB - Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non-anaplastic, four diffuse/anaplastic, three nodular/non-anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl-2, and Ki-67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non-anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl-2. In addition, Ki-67 labeling indices for anaplastic subtypes (39.0 ± 7.42%) were significantly higher than that of non-anaplastic medulloblastomas (11.4 ± 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.

KW - Anaplasia

KW - Histopathology

KW - Immunohistochemistry

KW - Medulloblastoma

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KW - Subtype

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Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas

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