Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction

Ekyune Kim; Ki Eun Park; Ji Su Kim; Dong Chul Baek; Jae Woong Lee; Sang Rae Lee; Myeong Su Kim; Sang Hyun Kim; Chan Shick Kim; Deog Bon Koo; Han Seok Kang; Zae Young Ryoo; Kyu Tae Chang

doi:10.1262/jrd.20134

Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction

Ekyune Kim, Ki Eun Park, Ji Su Kim, Dong Chul Baek, Jae Woong Lee, Sang Rae Lee, Myeong Su Kim, Sang Hyun Kim, Chan Shick Kim, Deog Bon Koo, Han Seok Kang, Zae Young Ryoo, Kyu Tae Chang

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.

Original language	English
Pages (from-to)	156-162
Number of pages	7
Journal	Journal of Reproduction and Development
Volume	55
Issue number	2
DOIs	https://doi.org/10.1262/jrd.20134
State	Published - 13 May 2009

Keywords

A disintegrin and metalloprotease (ADAM)
Disintegrin domain
Porcine
Sperm-egg interaction

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10.1262/jrd.20134

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title = "Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction",

abstract = "In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.",

keywords = "A disintegrin and metalloprotease (ADAM), Disintegrin domain, Porcine, Sperm-egg interaction",

author = "Ekyune Kim and Park, \{Ki Eun\} and Kim, \{Ji Su\} and Baek, \{Dong Chul\} and Lee, \{Jae Woong\} and Lee, \{Sang Rae\} and Kim, \{Myeong Su\} and Kim, \{Sang Hyun\} and Kim, \{Chan Shick\} and Koo, \{Deog Bon\} and Kang, \{Han Seok\} and Ryoo, \{Zae Young\} and Chang, \{Kyu Tae\}",

year = "2009",

month = may,

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doi = "10.1262/jrd.20134",

language = "English",

volume = "55",

pages = "156--162",

journal = "Journal of Reproduction and Development",

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publisher = "The Japanese Society of Animal Reproduction (JSAR)",

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T1 - Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction

AU - Kim, Ekyune

AU - Park, Ki Eun

AU - Kim, Ji Su

AU - Baek, Dong Chul

AU - Lee, Jae Woong

AU - Lee, Sang Rae

AU - Kim, Myeong Su

AU - Kim, Sang Hyun

AU - Kim, Chan Shick

AU - Koo, Deog Bon

AU - Kang, Han Seok

AU - Ryoo, Zae Young

AU - Chang, Kyu Tae

PY - 2009/5/13

Y1 - 2009/5/13

N2 - In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.

AB - In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.

KW - A disintegrin and metalloprotease (ADAM)

KW - Disintegrin domain

KW - Porcine

KW - Sperm-egg interaction

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SN - 0916-8818

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EP - 162

JO - Journal of Reproduction and Development

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Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction

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Keywords

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