TY - JOUR
T1 - Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry
AU - Islam, Syful
AU - Alam, Rafiqul
AU - Kim, Sunghwan
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2022/3/30
Y1 - 2022/3/30
N2 - In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase.
AB - In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase.
KW - Centella asiatica
KW - Laser desorption/ionization
KW - Metabolite profiling
KW - Solid-phase
KW - Tandem mass spectrometry (MS/MS)
UR - http://www.scopus.com/inward/record.url?scp=85119597060&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2021.131541
DO - 10.1016/j.foodchem.2021.131541
M3 - Article
C2 - 34810014
AN - SCOPUS:85119597060
SN - 0308-8146
VL - 373
JO - Food Chemistry
JF - Food Chemistry
M1 - 131541
ER -