Improved marker-assisted selection efficiency of multi-resistance in doubled haploid rice plants

During rice cultivation, brown planthopper (BPH, Nilaparvata lugens Stal.) and white-backed planthopper (WBPH, Sogatella furcifera) infestations cause decreased yield and rice quality. In this study, we attempted to develop multi-resistance plants and increase the marker-assisted selection (MAS) efficiency. The frequency of callus induction in 95 F₁ doubled haploid lines (DHLs) derived from JSNDH13 (BPH resistance)/CNDH32 (WBPH resistance) ranged from 3.8 to 15.2 %. The plant regeneration rates varied from 2.9 to 39.3 %. Selected 75 DH plants were used to compare the results of WBPH bioassay and DNA marker reaction. The DHL bioassay showed continuous gene distribution and resistance score ranged from 0.5 to 9.0 (mean, 4.2). The broad-sense heritability for WBPH resistance was 51.7 %. The DHLs from the cross of JSNDH13/CNDH32 were segregated into 39 resistant (R):36 susceptible (S) to WBPH, i.e., 1 RR:SS. For the bioassay and MAS, RM11669 with WBPH resistance was selected. Genetically, the use of resistant cultivars, MAS, and DH breeding has proven to be environment friendly, efficient, and economical. This study confirmed the genetic basis for MAS to improve WBPH resistance in DHLs of WBPH/BPH resistant rice plants.