In vitro apoptotic effect of cassaine-type diterpene amides from Erythrophleum fordii on PC-3 prostate cancer cells

Tran Manh Hung, To Dao Cuong, Jeong Ah Kim, Jeong Hyung Lee, Mi Hee Woo, Byung Sun Min

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Cytotoxic activity-guided fractionation of Erythrophleum fordii led to the isolation of two new cassaine diterpenoid-diterpenoid amide dimers, erythrophlesins H-I (1, 2). Spectral data indicated that they consist of asymmetrical dimeric structure via an ester bond between two cassaine diterpenoids. MTT assay confirmed that compound 1, erythrophlesin H, had the strongest cytotoxic effect toward the human prostate cancer cell line, PC-3. The molecular mechanism by which this compound induced apoptosis cell in prostate cancer remains unknown. Erythrophlesin H induced apoptosis in a dose-dependent manner. Acridine orange and annexin V-FITC/PI double staining confirmed that erythrophlesin H effectively induces apoptosis in PC-3 cells.

Original languageEnglish
Pages (from-to)4989-4994
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume24
Issue number21
DOIs
StatePublished - 1 Nov 2014

Keywords

  • Cassaine diterpenoid amide
  • Erythrophlesin H
  • Erythrophleum fordii
  • Leguminosae
  • PC-3 apoptosis

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