In Vitro Dual Detection of GNPs Conjugated Rabbit IgG Using Anti-IgG Anchored Calcein Green Fluorescent LC Microdroplets

Kyubae Lee; Hanbyeol Shin; Kailash Chandra Gupta; Dong Yun Lee; Soo Young Park; Inn Kyu Kang

doi:10.1109/JSEN.2018.2865017

In Vitro Dual Detection of GNPs Conjugated Rabbit IgG Using Anti-IgG Anchored Calcein Green Fluorescent LC Microdroplets

Kyubae Lee, Hanbyeol Shin, Kailash Chandra Gupta, Dong Yun Lee, Soo Young Park, Inn Kyu Kang

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The 4-cyano-4′-pentyl biphenyl (5CB) liquid crystals molecules are fascinating materials for the fabrication of sensors as they are able to show a detectable optical signal in presence of small variation in surface forces. The sensitivity of 5CB molecules increased further on using them as microdroplets emulsion as compared to layered structures on plannar solid surfaces. The anchoring of receptor (AIgG) on LC microdroplets makes microdroplets capable to produce species specific optical signal in presence of nonspecific interactions. Gold nanoparticles (GNPs) have increased the interactions of antigen (IgG) with LC microdroplets anchored AIgG. The concomitant emission of calcein green fluorescence on interactions of GNPs anchored esterase with LC microdroplet anchored calcein-AM has provided an evidence to confirm the interactions induced transition from radial to bipolar in LC microdroplets. The AIgG (4.2μg, 28 pmol) and calcein-AM (4.8μg, 4.55 nmol) anchored LC microdroplets within a size variation of 20-30μm have shown a lower limit of detection of 0.22 ± 0.001 pmol of IgG in PBS (pH 7.4) within a contact time of 20 minutes. Thus, liquid crystals microdroplets have sufficient scope to develop biosensors for in vitro detection of disease causing antigens in biological fluids.

Original language	English
Article number	8449986
Pages (from-to)	8208-8214
Number of pages	7
Journal	IEEE Sensors Journal
Volume	18
Issue number	20
DOIs	https://doi.org/10.1109/JSEN.2018.2865017
State	Published - 15 Oct 2018

Keywords

Antigen-antibody interactions
biosensor
calcein-AM
gold nanoparticles

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title = "In Vitro Dual Detection of GNPs Conjugated Rabbit IgG Using Anti-IgG Anchored Calcein Green Fluorescent LC Microdroplets",

abstract = "The 4-cyano-4′-pentyl biphenyl (5CB) liquid crystals molecules are fascinating materials for the fabrication of sensors as they are able to show a detectable optical signal in presence of small variation in surface forces. The sensitivity of 5CB molecules increased further on using them as microdroplets emulsion as compared to layered structures on plannar solid surfaces. The anchoring of receptor (AIgG) on LC microdroplets makes microdroplets capable to produce species specific optical signal in presence of nonspecific interactions. Gold nanoparticles (GNPs) have increased the interactions of antigen (IgG) with LC microdroplets anchored AIgG. The concomitant emission of calcein green fluorescence on interactions of GNPs anchored esterase with LC microdroplet anchored calcein-AM has provided an evidence to confirm the interactions induced transition from radial to bipolar in LC microdroplets. The AIgG (4.2μg, 28 pmol) and calcein-AM (4.8μg, 4.55 nmol) anchored LC microdroplets within a size variation of 20-30μm have shown a lower limit of detection of 0.22 ± 0.001 pmol of IgG in PBS (pH 7.4) within a contact time of 20 minutes. Thus, liquid crystals microdroplets have sufficient scope to develop biosensors for in vitro detection of disease causing antigens in biological fluids.",

keywords = "Antigen-antibody interactions, biosensor, calcein-AM, gold nanoparticles",

author = "Kyubae Lee and Hanbyeol Shin and Gupta, {Kailash Chandra} and Lee, {Dong Yun} and Park, {Soo Young} and Kang, {Inn Kyu}",

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AU - Park, Soo Young

AU - Kang, Inn Kyu

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In Vitro Dual Detection of GNPs Conjugated Rabbit IgG Using Anti-IgG Anchored Calcein Green Fluorescent LC Microdroplets

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