In vitro metabolism study of seongsanamide a in human liver microsomes using non-targeted metabolomics and feature-based molecular networking

Zhexue Wu, Geum Jin Kim, So Young Park, Jong Cheol Shon, Kwang Hyeon Liu, Hyukjae Choi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Seongsanamide A is a bicyclic peptide with an isodityrosine residue discovered in Bacillus safensis KCTC 12796BP which exhibits anti-allergic activity in vitro and in vivo without significant cytotoxicity. The purpose of this study was to elucidate the in vitro metabolic pathway and potential for drug interactions of seongsanamide A in human liver microsomes using non-targeted metabolomics and feature-based molecular networking (FBMN) techniques. We identified four metabolites, and their structures were elucidated by interpretation of high-resolution tandem mass spectra. The primary metabolic pathway associated with seongsanamide A metabolism was hy-droxylation and oxidative hydrolysis. A reaction phenotyping study was also performed using recombinant cytochrome P450 isoforms. CYP3A4 and CYP3A5 were identified as the major metabolic enzymes responsible for metabolite formation. Seongsanamide A did not inhibit the cytochrome P450 isoforms commonly involved in drug metabolism (IC50 > 10 µM). These results will contribute to further understanding the metabolism and drug interaction potential of various bicyclic peptides.

Original languageEnglish
Article number1031
JournalPharmaceutics
Volume13
Issue number7
DOIs
StatePublished - Jul 2021

Keywords

  • Bacillus sp. KCTC 12796BP
  • Drug interaction
  • High-resolution mass spec-trometry
  • Metabolism
  • Seongsanamide A

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