In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera

Hyung Jin Lim; Yeong Seon Won; Seon Gyeong Bak; Soyoung Lee; Bomi Ryu; Seon Heui Cha; Tae Ho Kwon; Kyu Suk Shim; Jisu Jeon; Hyuck Se Kwon; Seung Woong Lee; Seung Jae Lee; Mun Chual Rho

doi:10.37290/ctnr2641-452X.21:60-65

In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera

Hyung Jin Lim
, Yeong Seon Won
, Seon Gyeong Bak
, Soyoung Lee
, Bomi Ryu
, Seon Heui Cha
, Tae Ho Kwon
, Kyu Suk Shim
, Jisu Jeon
, Hyuck Se Kwon
, Seung Woong Lee
, Seung Jae Lee
, Mun Chual Rho

Department of Innovative Pharmaceutical Sciences

Korea Research Institute of Bioscience and Biotechnology
Pukyong National University
Hanseo University
University of Science and Technology UST
Univera Co., Ltd.
Ltd.
Jeonbuk National University

Research output: Contribution to journal › Article › peer-review

Abstract

We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.

Original language	English
Pages (from-to)	60-65
Number of pages	6
Journal	Current Topics in Nutraceutical Research
Volume	21
Issue number	1
DOIs	https://doi.org/10.37290/ctnr2641-452X.21:60-65
State	Published - Feb 2023

Keywords

Aloe vera
Antibiotic resistance
IL-6/STAT3
Lactococcus lactis subsp. hordniae
Probiotic characteristics
Probiotics

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10.37290/ctnr2641-452X.21:60-65

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Lim, H. J., Won, Y. S., Bak, S. G., Lee, S., Ryu, B., Cha, S. H., Kwon, T. H., Shim, K. S., Jeon, J., Kwon, H. S., Lee, S. W., Lee, S. J., & Rho, M. C. (2023). In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera. Current Topics in Nutraceutical Research, 21(1), 60-65. https://doi.org/10.37290/ctnr2641-452X.21:60-65

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abstract = "We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.",

keywords = "Aloe vera, Antibiotic resistance, IL-6/STAT3, Lactococcus lactis subsp. hordniae, Probiotic characteristics, Probiotics",

author = "Lim, \{Hyung Jin\} and Won, \{Yeong Seon\} and Bak, \{Seon Gyeong\} and Soyoung Lee and Bomi Ryu and Cha, \{Seon Heui\} and Kwon, \{Tae Ho\} and Shim, \{Kyu Suk\} and Jisu Jeon and Kwon, \{Hyuck Se\} and Lee, \{Seung Woong\} and Lee, \{Seung Jae\} and Rho, \{Mun Chual\}",

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doi = "10.37290/ctnr2641-452X.21:60-65",

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T1 - In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera

AU - Lim, Hyung Jin

AU - Won, Yeong Seon

AU - Bak, Seon Gyeong

AU - Lee, Soyoung

AU - Ryu, Bomi

AU - Cha, Seon Heui

AU - Kwon, Tae Ho

AU - Shim, Kyu Suk

AU - Jeon, Jisu

AU - Kwon, Hyuck Se

AU - Lee, Seung Woong

AU - Lee, Seung Jae

AU - Rho, Mun Chual

PY - 2023/2

Y1 - 2023/2

N2 - We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.

AB - We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.

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KW - Antibiotic resistance

KW - IL-6/STAT3

KW - Lactococcus lactis subsp. hordniae

KW - Probiotic characteristics

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ER -

In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera

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