In vitro selection and rapid screening of high-lysine mutants in rice

G. H. Yi; M. H. Nam; B. G. Oh; H. Y. Kim

doi:10.1142/9789812814319_0201

In vitro selection and rapid screening of high-lysine mutants in rice

G. H. Yi, M. H. Nam, B. G. Oh, H. Y. Kim

Department of Farm Management

Rural Development Administration

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Lysine is the first limiting essential amino acid for optimal nutritional quality in rice grain. Somaclonal variation was the source of mutation in our breeding program. Biochemical selection was conducted using 1 mM S-(2-aminoethyl) cysteine followed by 5 mM lysine plus threonine in the callus subculture medium. The lysine content in the endosperm of all progenies regenerated from the surviving calli was higher than that of their donor cultivars. These elevated lysine levels of mutants were successfully transmitted to the M₄ generation. Absorbance at 570 nm in the ninhydrin color test was significantly correlated with lysine content and the composition of rice endosperm.

Original language	English
Title of host publication	Advances In Rice Genetics (In 2 Parts)
Publisher	World Scientific Publishing Co.
Pages	526-527
Number of pages	2
ISBN (Electronic)	9789812814319
DOIs	https://doi.org/10.1142/9789812814319_0201
State	Published - 1 Jan 2008

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title = "In vitro selection and rapid screening of high-lysine mutants in rice",

abstract = "Lysine is the first limiting essential amino acid for optimal nutritional quality in rice grain. Somaclonal variation was the source of mutation in our breeding program. Biochemical selection was conducted using 1 mM S-(2-aminoethyl) cysteine followed by 5 mM lysine plus threonine in the callus subculture medium. The lysine content in the endosperm of all progenies regenerated from the surviving calli was higher than that of their donor cultivars. These elevated lysine levels of mutants were successfully transmitted to the M4 generation. Absorbance at 570 nm in the ninhydrin color test was significantly correlated with lysine content and the composition of rice endosperm.",

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AU - Kim, H. Y.

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N2 - Lysine is the first limiting essential amino acid for optimal nutritional quality in rice grain. Somaclonal variation was the source of mutation in our breeding program. Biochemical selection was conducted using 1 mM S-(2-aminoethyl) cysteine followed by 5 mM lysine plus threonine in the callus subculture medium. The lysine content in the endosperm of all progenies regenerated from the surviving calli was higher than that of their donor cultivars. These elevated lysine levels of mutants were successfully transmitted to the M4 generation. Absorbance at 570 nm in the ninhydrin color test was significantly correlated with lysine content and the composition of rice endosperm.

AB - Lysine is the first limiting essential amino acid for optimal nutritional quality in rice grain. Somaclonal variation was the source of mutation in our breeding program. Biochemical selection was conducted using 1 mM S-(2-aminoethyl) cysteine followed by 5 mM lysine plus threonine in the callus subculture medium. The lysine content in the endosperm of all progenies regenerated from the surviving calli was higher than that of their donor cultivars. These elevated lysine levels of mutants were successfully transmitted to the M4 generation. Absorbance at 570 nm in the ninhydrin color test was significantly correlated with lysine content and the composition of rice endosperm.

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BT - Advances In Rice Genetics (In 2 Parts)

PB - World Scientific Publishing Co.

ER -

In vitro selection and rapid screening of high-lysine mutants in rice

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