Indoleacetic acid production and plant growth promoting potential of bacterial endophytes isolated from rice (Oryza sativa L.) seeds

Raheem Shahzad, Muhammad Waqas, Abdul Latif Khan, Khadua Al-Hosni, Sang Mo Kang, Chang Woo Seo, In Jung Lee

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Bacterial endophytes from the phyllosphere and rhizosphere have been used to produce bioactive metabolites and to promote plant growth. However, little is known about the endophytes residing in seeds. This study aimed to isolate and identify seed-borne bacterial endophytes from rice and elucidate their potential for phytohormone production and growth enhancement. The isolated endophytes included Micrococcus yunnanensis RWL-2, Micrococcus luteus RWL-3, Enterobacter soli RWL-4, Leclercia adecarboxylata RWL-5, Pantoea dispersa RWL-6, and Staphylococcus epidermidis RWL-7, which were identified using 16S rRNA sequencing and phylogenetic analysis. These strains were analyzed for indoleacetic acid (IAA) production by using GC-MS and IAA was found in the range of 11.50±0.77 μg ml-1 to 38.80± 1.35 Hg ml-1. We also assessed the strains for plant growth promoting potential because these isolates were able to produce IAA in pure culture. Most of the growth attributes of rice plants (shoot and root length, fresh and dry biomass, and chlorophyll content) were significantly increased by bacterial endophytes compared to the controls. These results show that IAA producing bacterial endophytes can improve hostplant growth traits and can be used as bio-fertilizers.

Original languageEnglish
Pages (from-to)175-186
Number of pages12
JournalActa Biologica Hungarica
Volume68
Issue number2
DOIs
StatePublished - Jun 2017

Keywords

  • Bio-fertilizers
  • Growth promotion
  • Iaa production
  • Rice
  • Seed-born bacterial endophytes

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