Infectious in vivo transcripts from a full-length clone of soybean mosaic virus strain G5H

Jang Kyun Seo; Hyeok Geun Lee; Hong Soo Choi; Su Heon Lee; Kook Hyung Kim

doi:10.5423/PPJ.2009.25.1.054

Infectious in vivo transcripts from a full-length clone of soybean mosaic virus strain G5H

Jang Kyun Seo, Hyeok Geun Lee, Hong Soo Choi, Su Heon Lee, Kook Hyung Kim

Department of Plant Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

An infectious full-length clone of Soybean mosaic vims (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-1 showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.

Original language	English
Pages (from-to)	54-61
Number of pages	8
Journal	Plant Pathology Journal
Volume	25
Issue number	1
DOIs	https://doi.org/10.5423/PPJ.2009.25.1.054
State	Published - 2009

Keywords

DNA-based vector
Infectious clone
SMV

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10.5423/PPJ.2009.25.1.054

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title = "Infectious in vivo transcripts from a full-length clone of soybean mosaic virus strain G5H",

abstract = "An infectious full-length clone of Soybean mosaic vims (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-1 showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.",

keywords = "DNA-based vector, Infectious clone, SMV",

author = "Seo, {Jang Kyun} and Lee, {Hyeok Geun} and Choi, {Hong Soo} and Lee, {Su Heon} and Kim, {Kook Hyung}",

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doi = "10.5423/PPJ.2009.25.1.054",

language = "English",

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AU - Seo, Jang Kyun

AU - Lee, Hyeok Geun

AU - Choi, Hong Soo

AU - Lee, Su Heon

AU - Kim, Kook Hyung

PY - 2009

Y1 - 2009

N2 - An infectious full-length clone of Soybean mosaic vims (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-1 showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.

AB - An infectious full-length clone of Soybean mosaic vims (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-1 showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.

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Infectious in vivo transcripts from a full-length clone of soybean mosaic virus strain G5H

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Keywords

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