Inhibitory effect of thrombin on the expression of secretory group IIA phospholipase A 2

Jong Sup Bae

doi:10.1002/jcb.23172

Inhibitory effect of thrombin on the expression of secretory group IIA phospholipase A ₂

Jong Sup Bae

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

It is well known that the expression level of secretory group IIA phospholipase A ₂ (sPLA ₂-IIA) is elevated in inflammatory diseases and lipopolysaccharide (LPS) up-regulates the expression of sPLA ₂-IIA in human umbilical vein endothelial cells (HUVECs). Recently, lower concentration thrombin could elicit anti-inflammatory responses in HUVECs. Here, the effects of lower concentration thrombin on the expression of sPLA ₂-IIA in LPS-stimulated HUVECs were investigated. Prior treatment of cells with thrombin (25-75pM) inhibited LPS-induced sPLA ₂-IIA expression by activating its receptor, protease-activated receptor-1 (PAR-1). And pretreatment of cells with either PI3-kinase inhibitor (LY294002) or cholesterol depleting agent (methyl-β-cyclodextrin, MβCD) abolished the inhibitory activity of thrombin against sPLA ₂-IIA expression. Therefore, these results suggest that PAR-1 activation by lower concentration thrombin inhibited LPS mediated expression of sPLA ₂-IIA by PAR-1 and PI3-kinase-dependent manner in lipid raft on the HUVECs.

Original language	English
Pages (from-to)	2502-2507
Number of pages	6
Journal	Journal of Cellular Biochemistry
Volume	112
Issue number	9
DOIs	https://doi.org/10.1002/jcb.23172
State	Published - Sep 2011

Keywords

HUVEC
Lipid raft
PAR-1
sPLA -IIA
Thrombin

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jcb.23172

Cite this

@article{b064d18bc3fc4e40824917d8dc1c284a,

title = "Inhibitory effect of thrombin on the expression of secretory group IIA phospholipase A 2 ",

abstract = "It is well known that the expression level of secretory group IIA phospholipase A 2 (sPLA 2-IIA) is elevated in inflammatory diseases and lipopolysaccharide (LPS) up-regulates the expression of sPLA 2-IIA in human umbilical vein endothelial cells (HUVECs). Recently, lower concentration thrombin could elicit anti-inflammatory responses in HUVECs. Here, the effects of lower concentration thrombin on the expression of sPLA 2-IIA in LPS-stimulated HUVECs were investigated. Prior treatment of cells with thrombin (25-75pM) inhibited LPS-induced sPLA 2-IIA expression by activating its receptor, protease-activated receptor-1 (PAR-1). And pretreatment of cells with either PI3-kinase inhibitor (LY294002) or cholesterol depleting agent (methyl-β-cyclodextrin, MβCD) abolished the inhibitory activity of thrombin against sPLA 2-IIA expression. Therefore, these results suggest that PAR-1 activation by lower concentration thrombin inhibited LPS mediated expression of sPLA 2-IIA by PAR-1 and PI3-kinase-dependent manner in lipid raft on the HUVECs.",

keywords = "HUVEC, Lipid raft, PAR-1, sPLA -IIA, Thrombin",

author = "Bae, {Jong Sup}",

year = "2011",

month = sep,

doi = "10.1002/jcb.23172",

language = "English",

volume = "112",

pages = "2502--2507",

journal = "Journal of Cellular Biochemistry",

issn = "0730-2312",

publisher = "Wiley-Liss Inc.",

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}

TY - JOUR

T1 - Inhibitory effect of thrombin on the expression of secretory group IIA phospholipase A 2

AU - Bae, Jong Sup

PY - 2011/9

Y1 - 2011/9

N2 - It is well known that the expression level of secretory group IIA phospholipase A 2 (sPLA 2-IIA) is elevated in inflammatory diseases and lipopolysaccharide (LPS) up-regulates the expression of sPLA 2-IIA in human umbilical vein endothelial cells (HUVECs). Recently, lower concentration thrombin could elicit anti-inflammatory responses in HUVECs. Here, the effects of lower concentration thrombin on the expression of sPLA 2-IIA in LPS-stimulated HUVECs were investigated. Prior treatment of cells with thrombin (25-75pM) inhibited LPS-induced sPLA 2-IIA expression by activating its receptor, protease-activated receptor-1 (PAR-1). And pretreatment of cells with either PI3-kinase inhibitor (LY294002) or cholesterol depleting agent (methyl-β-cyclodextrin, MβCD) abolished the inhibitory activity of thrombin against sPLA 2-IIA expression. Therefore, these results suggest that PAR-1 activation by lower concentration thrombin inhibited LPS mediated expression of sPLA 2-IIA by PAR-1 and PI3-kinase-dependent manner in lipid raft on the HUVECs.

AB - It is well known that the expression level of secretory group IIA phospholipase A 2 (sPLA 2-IIA) is elevated in inflammatory diseases and lipopolysaccharide (LPS) up-regulates the expression of sPLA 2-IIA in human umbilical vein endothelial cells (HUVECs). Recently, lower concentration thrombin could elicit anti-inflammatory responses in HUVECs. Here, the effects of lower concentration thrombin on the expression of sPLA 2-IIA in LPS-stimulated HUVECs were investigated. Prior treatment of cells with thrombin (25-75pM) inhibited LPS-induced sPLA 2-IIA expression by activating its receptor, protease-activated receptor-1 (PAR-1). And pretreatment of cells with either PI3-kinase inhibitor (LY294002) or cholesterol depleting agent (methyl-β-cyclodextrin, MβCD) abolished the inhibitory activity of thrombin against sPLA 2-IIA expression. Therefore, these results suggest that PAR-1 activation by lower concentration thrombin inhibited LPS mediated expression of sPLA 2-IIA by PAR-1 and PI3-kinase-dependent manner in lipid raft on the HUVECs.

KW - HUVEC

KW - Lipid raft

KW - PAR-1

KW - sPLA -IIA

KW - Thrombin

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