TY - JOUR
T1 - Inhibitory effects of oleanane-type triterpenes and saponins from the stem bark of Kalopanax pictus on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells
AU - Quang, Tran Hong
AU - Ngan, Nguyen Thi Thanh
AU - Van Minh, Chau
AU - Van Kiem, Phan
AU - Nhiem, Nguyen Xuan
AU - Tai, Bui Huu
AU - Thao, Nguyen Phuong
AU - Chae, Doobyeong
AU - Mathema, Vivek Bhakta
AU - Koh, Young Sang
AU - Lee, Je Hyun
AU - Yang, Seo Young
AU - Kim, Young Ho
PY - 2013/3
Y1 - 2013/3
N2 - Kalopanax pictus (Araliaceae) is a deciduous tree distributed in Korea, Japan, and China. The stem bark of K. pictus has been functionally used as a traditional crude drug for the treatment of various inflammatory diseases. In the present study, we describe the inhibitory effects of oleanane-type triterpenes and saponins isolated from the stem bark of K. pictus on production of pro-inflammatory cytokines in LPS-stimulated bone marrow-derived dendritic cells. Of the compounds tested, 16,23,29-trihydroxy-3-oxo-olean-12-en-28-oic acid (1), 4,23,29-trihydroxy-3,4-seco-olean-12-en-3-oate-28-oic acid (2), 3β,6β,23-trihydroxyolean-12-en-28-oic acid 28-O-β-D- glucopyranoside (3), nipponogenin E (6), 3β,6β,23-trihydroxyolean-12- en-28-oic acid (7), and caulophyllogenin (19) significantly inhibited the production of IL-12 p40 and IL-6 with IC50 values ranging from 3.3 to 9.1 μM. Compounds 2, 3, 7, and 19 significantly suppressed the secretion of TNF-α with IC50 ranging from 8.8 to 20.0 μM. These data provide scientific support for the use of K. pictus stem bark and its triterpene and saponin components in the inhibition of pro-inflammatory cytokine secretion, including IL-12 p40, IL-6, and TNF-α, and for prevention and treatment of inflammatory diseases.
AB - Kalopanax pictus (Araliaceae) is a deciduous tree distributed in Korea, Japan, and China. The stem bark of K. pictus has been functionally used as a traditional crude drug for the treatment of various inflammatory diseases. In the present study, we describe the inhibitory effects of oleanane-type triterpenes and saponins isolated from the stem bark of K. pictus on production of pro-inflammatory cytokines in LPS-stimulated bone marrow-derived dendritic cells. Of the compounds tested, 16,23,29-trihydroxy-3-oxo-olean-12-en-28-oic acid (1), 4,23,29-trihydroxy-3,4-seco-olean-12-en-3-oate-28-oic acid (2), 3β,6β,23-trihydroxyolean-12-en-28-oic acid 28-O-β-D- glucopyranoside (3), nipponogenin E (6), 3β,6β,23-trihydroxyolean-12- en-28-oic acid (7), and caulophyllogenin (19) significantly inhibited the production of IL-12 p40 and IL-6 with IC50 values ranging from 3.3 to 9.1 μM. Compounds 2, 3, 7, and 19 significantly suppressed the secretion of TNF-α with IC50 ranging from 8.8 to 20.0 μM. These data provide scientific support for the use of K. pictus stem bark and its triterpene and saponin components in the inhibition of pro-inflammatory cytokine secretion, including IL-12 p40, IL-6, and TNF-α, and for prevention and treatment of inflammatory diseases.
KW - Araliaceae
KW - IL-12 p40
KW - IL-6
KW - Kalopanax pictus
KW - LPS-stimulated BMDC
KW - Oleanane-type triterpene
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=84879554358&partnerID=8YFLogxK
U2 - 10.1007/s12272-013-0031-8
DO - 10.1007/s12272-013-0031-8
M3 - Article
C2 - 23444041
AN - SCOPUS:84879554358
SN - 0253-6269
VL - 36
SP - 327
EP - 334
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
IS - 3
ER -