Inhibitory Effects of Plant-Derived Flavonoids and Phenolic Acids on Malonaldehyde Formation from Ethyl Arachidonate

Kwang Geun Lee, Takayuki Shibamoto, Gary R. Takeoka, Sung Eun Lee, Jeong Han Kim, Byeoung Soo Park

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The antioxidant activities of naturally occurring plant compounds were measured in a lipid peroxidation system consisting of ethyl arachidonate and Fenton's reagent. Inhibitory effects of 24 plant-derived flavonoids and 5 phenolic acids on malonaldehyde (MA) formation from ethyl arachidonate were examined using gas chromatography (GC) with a nitrogen-phosphorus detector (NPD). Luteolin, which showed the strongest antioxidant activity, inhibited MA formation by 94% and 97% at the levels of 0.5 and 1.0 mM, respectively. The antioxidant activities of the flavones and flavonols decreased in the following order: luteolin > rhamnetin > fisetin > kaempferol > morin > quercetin. Among the flavanones tested, hesperitin, taxifolin, and naringenin exhibited appreciable antioxidant activities (61-84%) at the 1.0 mM level. The inhibitory effect of epigallocatechin gallate (82.5% at the 1.0 mM level) was the strongest among the flavan-3-ols tested. Ferulic acid had the most potent antioxidant activity (74.6% at the 1.0 mM level) of the phenolic acids tested.

Original languageEnglish
Pages (from-to)7203-7207
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number24
DOIs
StatePublished - 19 Nov 2003

Keywords

  • Flavonoids
  • Lipid peroxidation
  • Malonaldehyde
  • Natural antioxidants

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