Inhibitory Effects of seco-Triterpenoids from Acanthopanax sessiliflorus Fruits on HUVEC Invasion and ACE Activity

This study was conducted to investigate the effects of the crude extract from Acanthopanax sessiliflorus fruits and the isolated seco-triterpenoids from the crude extract on blood flow in human umbilical vein endothelial cell (HUVEC) invasion assay and angiotensin converting enzyme (ACE) inhibitory activity assay. On the basis of DMSO, the extent of HUVECs'invasion was remarkably decreased with crude extract concentrations of 400 and 1000 μg/mL. Additionally, the extent of the HUVEC invasion inhibitory effect in 400 and 1000 μg/mL of acanthosessilioside F were 55.8% and 72.4%, respectively. In addition, the maximum extent of the HUVEC invasion inhibitory effect of 22-α-hydroxychiisanoside was 88.9%. The IC₅₀ value of the inhibitory effect on ACE activity in the crude extract was 4 μg/mL. The isolated seco-triterpenoids, 22α-hydroxychiisanogenin, 3,4-seco-lupan-20(30)-en-3,28-dioic acid, (1R)-1,4-epoxy-11α,22α-hydroxy-3,4-seco-lupan-20(30)-en-3,28-dioicacid, (+)-divaroside, and chiisanosidehad showed very high inhibitory effects on ACE activity, ranging from 1.8 to 2.9 üg/mL, which is much higher than the 150.0 üg/mL effect of aspirin. These results suggest that the crude extract from Acanthopanax sessiliflorus fruits and the isolated seco-triterpenoids from the crude extract enhance the blood flow effect by decreasing ACE activity.